Alternatively spliced transcripts of the Drosophila tramtrack gene encode zinc finger proteins with distinct DNA binding specificities.
Alternatively spliced transcripts of the Drosophila tramtrack gene encode zinc finger proteins with distinct DNA binding specificities.
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DOI：
10.1002/j.1460-2075.1992.tb05142.x
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发表时间：
1992-03
期刊：
影响因子：
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通讯作者：
D. Read;J. Manley
中科院分区：
文献类型：
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作者：
D. Read;J. Manley
果蝇胚胎核提取物中存在的一种蛋白质结合了启动子中的多个位点，并且配对规则基因的遗传定义的自动调节元件甚至跳过了（eve）。我们在这里报告的分离的cDNA编码这种结合活性，其序列识别它作为69 kDa的锌指tramtrack（ttk）蛋白。由于ttk之前参与控制另一个配对规则基因fushi tarazu（ftz）的表达，我们的研究结果表明ttk在至少两个发育重要基因的调节中发挥作用。分离了另外一个ttk相关cDNA克隆，其产生了一种88 kDa蛋白，该蛋白具有一组替代的锌指，其DNA结合特异性与69 kDa蛋白不同。这两种蛋白质都被证明是由ttk基因通过选择性剪接编码的，提供了使用这种机制来产生具有不同DNA结合特异性的相关蛋白质的第一个例子。整装原位杂交分析显示两种TTK mRNA亚型的胚胎表达模式不同。
A protein present in nuclear extracts of Drosophila embryos binds multiple sites in the promoter and genetically defined autoregulatory element of the pair‐rule gene even‐skipped (eve). We reported here the isolation of a cDNA encoding this binding activity, the sequence of which identifies it as the 69 kDa zinc finger tramtrack (ttk) protein. As ttk was previously implicated in controlling the expression of another pair‐rule gene, fushi tarazu (ftz), our findings suggest that ttk plays a role in the regulation of at least two developmentally important genes. An additional ttk‐related cDNA clone was isolated which gives rise to an 88 kDa protein with an alternative set of zinc fingers having a DNA binding specificity distinct from that of the 69 kDa protein. Both proteins were shown to be encoded by the ttk gene through alternative splicing, providing the first example of the use of this mechanism to generate related proteins with distinct DNA binding specificities. Whole mount in situ hybridization analysis revealed different patterns of embryonic expression of the two ttk mRNA isoforms.