Limiting impairment of muscle function following ischemia and reperfusion in rabbits.
Limiting impairment of muscle function following ischemia and reperfusion in rabbits.
复制标题
限制兔子缺血和再灌注后肌肉功能的损害。
DOI：
10.1055/s-2007-1006474
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发表时间：
1996
影响因子：
2.1
通讯作者：
Klitzman,B
中科院分区：
文献类型：
--
作者：
Zavitsanos,G;Huang,L;Panza,W;Serafin,D;Klitzman,B
再灌注损伤是微血管重建的复杂现象。文献中存在对自由基清除剂的广泛研究。维拉帕米，去铁胺和二甲亚砜的潜在有益作用进行了研究，在兔前肢进行3小时的止血带缺血，然后再灌注一段时间。在松开止血带前5分钟，对家兔单次静脉输注生理盐水、维拉帕米（0.2 mg/kg）、去铁胺（50 mg/kg）或二甲亚砜（100 mg/kg）。再灌注后，光学显微镜和激光多普勒血流仪均未显示假手术和治疗肢体之间存在显著差异。肌肉功能通过测量指浅屈肌的最大抽搐张力来评估。维拉帕米或去铁胺可显著改善再灌注后0.5、3和24小时肌肉收缩功能的恶化。相反，二甲亚砜使缺血后的肌肉功能恶化。二甲亚砜似乎没有益处，而维拉帕米和去铁胺可能有益于减轻微血管移植中的再灌注损伤。
Reperfusion injury is a phenomenon complicating microvascular reconstruction. Extensive investigations of free-radical scavengers exist in the literature. The potential beneficial effects of verapamil, deferox-amine, and dimethylsulfoxide were studied in the rabbit forelimb subjected to 3 hr of tourniquet ischemia, followed by a period of reperfusion. Five minutes prior to tourniquet release, rabbits were given single intravenous infusions of normal saline, verapamil (0.2 mg/kg), deferoxamine (50 mg/kg), or dimethylsulfoxide (100 mg/kg). Following reperfusion, neither light microscopy nor laser Doppler flowmetry revealed significant differences between the sham and treated limbs. Muscle function was evaluated by measuring maximal twitch tensions in the flexor digitorum superficialis. Deterioration of muscle contractile function at 0.5, 3, and 24 hr after reperfusion was significantly improved by treatment with verapamil or deferoxamine. Conversely, dimethylsulfoxide worsened muscle function post ischemia. Dimethylsulfoxide does not appear to be beneficial, while verapamil and deferoxamine may be of benefit in mitigating reperfusion injury in microvascular transplantation.