Photoacoustic microscopy using supercontinuum light for in vivo microscopic imaging of living tissue dynamics
Photoacoustic microscopy using supercontinuum light for in vivo microscopic imaging of living tissue dynamics
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DOI：
10.1117/12.2652280
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发表时间：
2023-03
期刊：
影响因子：
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通讯作者：
T. Hirasawa;Kazuyoshi Tachi;Manami Miyashita;T. Ishikawa;K Ito;M. Ishihara
中科院分区：
文献类型：
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作者：
T. Hirasawa;Kazuyoshi Tachi;Manami Miyashita;T. Ishikawa;K Ito;M. Ishihara
为了理解在活组织中发生的各种现象，需要使活组织中的细胞和分子的动态可视化的高速体积成像技术。为此，我们已经开发了一种反射MS-PAI与高激发波长的灵活性，使用超连续谱光源和带通滤波器，和超过100 fps的快速B-扫描速率。MS-PAM用于细胞动力学成像的能力通过对流过血管的造影剂染色的细胞进行延时体积成像来证明。我们计划将MS-PAM技术与功能化造影剂（成像探针）结合联合收割机来分析动态现象。
To understand various phenomena that occur in living tissue, high-speed volume imaging technology that visualizes the dynamics of cells and molecules in living tissue is desired. For this purpose, we have developed a reflective MS-PAI with high excitation wavelength flexibility using a supercontinuum light source and bandpass filters, and a fast B-scan rate of over 100 fps. The ability of the MS-PAM for cell dynamics imaging was demonstrated by performing time-lapse volume imaging of contrast agent-stained cells flowing through blood vessels. We plan to combine the MS-PAM technology with functionalized contrast agents (imaging probes) to analyze dynamic phenomena.