Altered lipid accumulation in Nannochloropsis salina CCAP849/3 following EMS and UV induced mutagenesis.
Altered lipid accumulation in Nannochloropsis salina CCAP849/3 following EMS and UV induced mutagenesis.
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DOI：
10.1016/j.btre.2015.05.007
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发表时间：
2015-09
期刊：
影响因子：
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通讯作者：
Ali ST
中科院分区：
文献类型：
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作者：
Beacham TA;Macia VM;Rooks P;White DA;Ali ST
纳米盐绿藻的EMS和UV诱变与FACS联合富集突变体。EMS突变体的生产力提高了76%，并显示出FA谱的变化范围。双EMS和UV突变体积累的脂质是野生型的3倍。脂质含量的升高伴随着影响生产力的增长率成本。适合不同行业产生的突变体（生物燃料，高价值PUFA生产）。微藻在一系列工业应用中具有作为化学原料的潜力。采用EMS诱变法对盐绿纳米藻进行诱变，并采用荧光活化细胞分选法筛选出含脂量最高的细胞。对生长、脂质含量和脂肪酸组成的评估发现，突变菌株表现出一系列改变的性状，包括PUFA含量的变化，总FAME比野生型菌株增加了156%。结合增长率的下降，这表明生产率提高了76%。紫外诱变后，突变培养物的脂质积累量增加到野生型菌株的3倍以上，但生长速度降低导致总体产量下降。观察到的变化表明ω - 6肯尼迪通路的调节发生了变化。讨论了这些生理变化对生物燃料生产等工业应用的重要性。
EMS and UV mutagenesis of Nannochloropsis salina combined with FACS for mutant enrichment. Productivity of EMS mutants increased by 76% and showed range of FA profile changes. Dual EMS and UV mutants accumulated 3 fold more lipid than the wild type. Elevation in lipid content comes with a cost to growth rate impacting productivity. Mutants suitable for divergent industries generated (biofuel, high value PUFA production). Microalgae have potential as a chemical feed stock in a range of industrial applications. Nannochloropsis salina was subject to EMS mutagenesis and the highest lipid containing cells selected using fluorescence-activated cell sorting. Assessment of growth, lipid content and fatty acid composition identified mutant strains displaying a range of altered traits including changes in the PUFA content and a total FAME increase of up to 156% that of the wild type strain. Combined with a reduction in growth this demonstrated a productivity increase of up to 76%. Following UV mutagenesis, lipid accumulation of the mutant cultures was elevated to more than 3 fold that of the wild type strain, however reduced growth rates resulted in a reduction in overall productivity. Changes observed are indicative of alterations to the regulation of the omega 6 Kennedy pathway. The importance of these variations in physiology for industrial applications such as biofuel production is discussed.