Nestin-positive progenitor cells derived from adult human pancreatic islets of Langerhans contain side population (SP) cells defined by expression of the ABCG2 (BCRP1) ATP-binding cassette transporter
Nestin-positive progenitor cells derived from adult human pancreatic islets of Langerhans contain side population (SP) cells defined by expression of the ABCG2 (BCRP1) ATP-binding cassette transporter
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DOI：
10.1016/s0006-291x(02)00275-9
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发表时间：
2002-05-03
影响因子：
3.1
通讯作者：
Habener, JF
中科院分区：
文献类型：
--
作者：
Lechner, A;Leech, CA;Habener, JF
糖尿病是由于胰腺朗格汉斯胰岛的p细胞不能产生满足身体需要的胰岛素而引起的。重度糖尿病患者全胰腺或胰岛移植可有效恢复胰岛素分泌。由于供体胰腺缺乏，需要开发替代胰岛来源，如离体培养和干细胞/祖细胞的分化。早些时候，我们在从成人胰腺分离的胰岛中发现了表达神经干细胞标记物巢蛋白的多潜能祖细胞：巢蛋白阳性的胰岛衍生祖细胞（NIPs）。最近有研究表明，确定造血干细胞多能侧群（SP）的Hoechst 33342染料的排除是由atp结合盒转运体ABCG2介导的。在这里，我们报道了人类胰岛源性NIPs包含大量共同表达ABCG2， MDR1的SP细胞亚群。巢蛋白。因此，NIPs可能是成体多能干细胞/祖细胞的潜在来源，可用于生产胰岛组织，用于移植到糖尿病患者体内。(C) 2002 Elsevier Science （USA）。版权所有。
The disease diabetes mellitus arises as a consequence of a failure of the P-cells in the islets of Langerhans of the pancreas to produce insulin in the amounts required to meet the needs of the body. Whole pancreas or islet transplants in patients with severe diabetes effectively restore insulin production. A lack of availability of donor pancreata requires the development of alternative sources of islets such as the ex vivo culture and differentiation of stem/progenitor cells. Earlier we discovered multipotential progenitor cells in islets isolated from adult human pancreata that express the neural stem cell marker nestin: nestin-positive islet-derived progenitor cells (NIPs). Recently it was shown that the exclusion of the Hoechst 33342 dye, which defines the pluripotential side population (SP) of hematopoietic stem cells, is mediated by the ATP-binding cassette transporter, ABCG2. Here we report that the human islet-derived NIPs contain a substantial subpopulation of SP cells that co-express ABCG2, MDR1. and nestin. Thus NIPs may be a potential source of adult pluripotential stem/progenitor cells useful for the production of islet tissue for transplantation into diabetic subjects. (C) 2002 Elsevier Science (USA). All rights reserved.