Peroxidase and phenylalanine ammonia-lyase activities, phenolic acid contents, and allelochemicals-inhibited root growth of soybean
Peroxidase and phenylalanine ammonia-lyase activities, phenolic acid contents, and allelochemicals-inhibited root growth of soybean
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DOI：
10.4067/s0716-97602002000100009
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发表时间：
2002-01-01
影响因子：
6.7
通讯作者：
FERRARESE-FILHO, OSVALDO
中科院分区：
文献类型：
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作者：
HERRIG, VANESSA;FERRARESE, MARIA DE LOURDES L.;FERRARESE-FILHO, OSVALDO
研究了化感物质阿魏酸（FA）和香草酸（VA）对大豆（Glycine max（L.）4.3.1.5 1.11.1.7梅尔）进行了检查。将3日龄的幼苗在含有FA或VA（0.1至1 mM）的营养液中培养48 h。两种化合物（0.5和1 mM）均降低根长（RL）、鲜重（FW）和干重（DW），并增加PhAs含量。在0.5和1 mM，FA增加可溶性POD活性（18%和47%，分别）和细胞壁（CW）结合POD活性（61%和34%），而VA增加可溶性POD活性（33%和17%），但不影响CW结合POD活性。在1 mM时，FA增加（82%），而VA降低（32%）PAL活性。结果进行了讨论的基础上，这些化合物的苯丙烷代谢和根生长的作用，并建议POD和PAL活性的影响所造成的一些化感物质影响植物生长的许多机制。
The influence of the allelochemicals ferulic (FA) and vanillic (VA) acids on peroxidase (POD, EC 1.11.1.7) and phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) activities and their relationships with phenolic acid (PhAs) contents and root growth of soybean (Glycine max (L.) Merr.) were examined. Three-day-old seedlings were cultivated in nutrient solution containing FA or VA (0.1 to 1 mM) for 48 h. Both compounds (at 0.5 and 1 mM) decreased root length (RL), fresh weight (FW) and dry weight (DW) and increased PhAs contents. At 0.5 and 1 mM, FA increased soluble POD activity (18% and 47%, respectively) and cell wall (CW)-bound POD activity (61% and 34%), while VA increased soluble POD activity (33% and 17%) but did not affect CW-bound POD activity. At I mM, FA increased (82%) while VA reduced (32%) PAL activities. The results are discussed on the basis of the role of these compounds on phenylpropanoid metabolism and root growth and suggest that the effects caused on POD and PAL activities are some of the many mechanisms by which allelochemicals influence plant growth.