Roadmaps and Detours: Active Chlorophyll- a Assessments of Primary Productivity Across Marine and Freshwater Systems.
Roadmaps and Detours: Active Chlorophyll- a Assessments of Primary Productivity Across Marine and Freshwater Systems.
复制标题
DOI：
10.1021/acs.est.8b03488
复制
发表时间：
2018-09
影响因子：
11.4
通讯作者：
David J. Hughes;Douglas Campbell;M. Doblin;J. Kromkamp;Evelyn Lawrenz;C. M. Moore;K. Oxborough;O. Prášil;Peter J. Ralph;Marco F. Alvarez;D. Suggett
中科院分区：
文献类型：
--
作者：
David J. Hughes;Douglas Campbell;M. Doblin;J. Kromkamp;Evelyn Lawrenz;C. M. Moore;K. Oxborough;O. Prášil;Peter J. Ralph;Marco F. Alvarez;D. Suggett
评估浮游植物在空间和时间上的生产力仍然是海洋学家和湖泊学家的核心目标。快速重复率荧光测定法（FRRf）以前所未有的分辨率和规模为实现这一目标提供了一种潜在的手段，尽管人们对此寄予厚望，但它尚未成为“首选”方法。一个主要障碍是难以将电子转移速率转换为与生物地球化学碳通量研究最相关的碳固定速率。这种困难源于方法上的不一致，以及我们对碳固定的电子需求（Φe，C）如何受到环境和浮游植物组合的组成和生理差异的影响的有限理解。我们概述了限制方法偏差的“路线图”，并对潜在的生态生理学有了更机械的理解Φe，C。我们1)重新评估控制微藻如何将光合电子传递衍生的能量和还原剂投入到储存的碳中，而不是替代碳汇的核心生理过程。然后，我们概述了促进FRRf更广泛的吸收和利用的步骤，这可能会改变我们对水生初级生产力的认识。我们认为现在是时候修改我们历史上把碳作为选择货币的方法，更好地理解电子传递从根本上驱动生态系统的生物地球化学，调节细胞间的相互作用，并最终改变群落的生物量和结构。
Assessing phytoplankton productivity over space and time remains a core goal for oceanographers and limnologists. Fast Repetition Rate fluorometry (FRRf) provides a potential means to realize this goal with unprecedented resolution and scale yet has not become the "go-to" method despite high expectations. A major obstacle is difficulty converting electron transfer rates to equivalent rates of C-fixation most relevant for studies of biogeochemical C-fluxes. Such difficulty stems from methodological inconsistencies and our limited understanding of how the electron requirement for C-fixation (Φe,C) is influenced by the environment and by differences in the composition and physiology of phytoplankton assemblages. We outline a "roadmap" for limiting methodological bias and to develop a more mechanistic understanding of the ecophysiology underlying Φe,C. We 1) re-evaluate core physiological processes governing how microalgae invest photosynthetic electron transport-derived energy and reductant into stored carbon versus alternative sinks. Then, we 2) outline steps to facilitate broader uptake and exploitation of FRRf, which could transform our knowledge of aquatic primary productivity. We argue it is time to 3) revise our historic methodological focus on carbon as the currency of choice, to 4) better appreciate that electron transport fundamentally drives ecosystem biogeochemistry, modulates cell-to-cell interactions, and ultimately modifies community biomass and structure.