ONE GENE, BUT 2 MESSENGER-RNAS ENCODE LIVER-L AND RED-CELL L' PYRUVATE-KINASE SUBUNITS
ONE GENE, BUT 2 MESSENGER-RNAS ENCODE LIVER-L AND RED-CELL L' PYRUVATE-KINASE SUBUNITS
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DOI：
10.1038/292070a0
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发表时间：
1981-01-01
期刊：
影响因子：
64.8
通讯作者：
KAHN, A
中科院分区：
文献类型：
--
作者：
MARIE, J;SIMON, MP;KAHN, A
红血球和肝脏的丙酮酸激酶亚基分子量不同。大鼠酶的多肽图谱显示，这种差异是由于红细胞酶中存在单一外显子多肽。有强有力的遗传学证据表明，这两种酶都是由同一结构基因编码的。利用从大鼠红细胞和肝脏中提取的RNA进行的体外蛋白质合成实验表明，这种差异反映在组织特异性mRNA上。因此，这种差异不是由于翻译后加工造成的，而是可能涉及基因重排或共同的核RNA前体的差异加工。
Pyruvate kinase subunits of red cells and liver differ in their molecular weights. Peptide mapping of the rat enzymes has shown that this difference is due to a single exon peptide present in the red cell enzyme. There is strong genetic evidence in man that both enzymes are encoded by the same structural gene. In vitro protein synthesis experiments using RNA extracted from rat red cells and liver demonstrated that the difference is reflected in tissue-specific mRNA. Thus the difference is not due to post-translational processing and presumably involves either gene rearrangement or differential processing of a common nuclear RNA precursor.