Identification of distinct layers within the stratified squamous epithelium of the adult human true vocal fold
Identification of distinct layers within the stratified squamous epithelium of the adult human true vocal fold
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DOI：
10.1002/lary.25264
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发表时间：
2015-09-01
期刊：
影响因子：
2.6
通讯作者：
Rajagopal, Jayaraj
中科院分区：
文献类型：
--
作者：
Dowdall, Jayme R.;Sadow, Peter M.;Rajagopal, Jayaraj
目标/假设缺乏对正常人声带上皮的不同细胞类型进行分类的精确分子模式。我们假设真正的声带上皮具有与其他复层鳞状上皮（包括皮肤、角膜、口腔粘膜和食道）相似的细胞结构和组织。与皮肤和胃肠道疾病类似，人类声带上皮内正常细胞类型的分子定义及其几何关系的描述应作为表征与化生、发育异常和癌症相关的细胞变化的基础。研究设计对成人喉进行定性研究。方法对正常人喉组织的组织学切片进行分析 形态学（苏木精和曙红）和免疫组织化学蛋白表达谱，包括细胞角蛋白（CK13 和 CK14）、角化包膜蛋白（外皮蛋白）、基底细胞（NGFR/p75）和增殖标记物（Ki67）。结果我们证明，具有独特标记谱的三种不同细胞层存在于复层鳞状上皮内。 真正的声带。我们使用这些定义来确定细胞增殖仅限于上皮内的某些细胞类型和层。这些不同的细胞类型在五个正常成人喉中是可重复的。结论我们已经确定，在真声襞的正常成人复层鳞状上皮内存在三层细胞。此外，复制的细胞群很大程度上局限于上皮内的副基底层。这种对不同细胞群的描述将有助于未来声带再生和癌症的研究。证据水平N/A。喉镜，125：E313-E319，2015
Objectives/HypothesisA precise molecular schema for classifying the different cell types of the normal human vocal fold epithelium is lacking. We hypothesize that the true vocal fold epithelium has a cellular architecture and organization similar to that of other stratified squamous epithelia including the skin, cornea, oral mucosa, and esophagus. In analogy to disorders of the skin and gastrointestinal tract, a molecular definition of the normal cell types within the human vocal fold epithelium and a description of their geometric relationships should serve as a foundation for characterizing cellular changes associated with metaplasia, dysplasia, and cancer.Study DesignQualitative study with adult human larynges.MethodsHistologic sections of normal human laryngeal tissue were analyzed for morphology (hematoxylin and eosin) and immunohistochemical protein expression profile, including cytokeratins (CK13 and CK14), cornified envelope proteins (involucrin), basal cells (NGFR/p75), and proliferation markers (Ki67).ResultsWe demonstrated that three distinct cell strata with unique marker profiles are present within the stratified squamous epithelium of the true vocal fold. We used these definitions to establish that cell proliferation is restricted to certain cell types and layers within the epithelium. These distinct cell types are reproducible across five normal adult larynges.ConclusionWe have established that three layers of cells are present within the normal adult stratified squamous epithelium of the true vocal fold. Furthermore, replicating cell populations are largely restricted to the parabasal strata within the epithelium. This delineation of distinct cell populations will facilitate future studies of vocal fold regeneration and cancer.Level of EvidenceN/A. Laryngoscope, 125:E313-E319, 2015