Bone marrow stromal cells inhibit caspase-12 expression in rats with spinal cord injury.
Bone marrow stromal cells inhibit caspase-12 expression in rats with spinal cord injury.
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骨髓基质细胞抑制脊髓损伤大鼠中caspase-12的表达
DOI：
10.3892/etm.2013.1201
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发表时间：
2013-09
影响因子：
2.7
通讯作者：
Wang L
中科院分区：
文献类型：
--
作者：
Liu W;Ding Y;Zhang X;Wang L
骨髓干细胞（BMSCs）对脊髓损伤（SCI）的潜在有益作用的机制尚不清楚。因此，本研究的目的是探讨骨髓间充质干细胞对大鼠脊髓损伤的保护作用。将45只成年雄性SD大鼠随机分为3组：脊髓损伤组（n=15）、骨髓间充质干细胞组（n=15）和假手术组（n=15）。在SCI和BMSC治疗组中，使用改良的艾伦重量下降技术诱导SCI。BMSC治疗组在损伤后24 h接受使用微针向脊髓中心注射BMSC。假手术组不做脊髓损伤，但切除相应椎板。移植后第7天，BMSC治疗组的BBB评分迅速恢复，而SCI组的BBB评分仍较低（P<0.05）。与假手术组相比，脊髓损伤组Caspase-12表达增加，而BMSC治疗组caspase-12表达减弱（P<0.05）。结论：骨髓间充质干细胞移植可改善脊髓损伤后大鼠的运动功能，降低caspase-12的表达。因此，它可能是预防脊髓严重损伤的有效策略。
The mechanisms underlying the potentially beneficial effect of bone marrow stem cells (BMSCs) on spinal cord injury (SCI) are unknown. Therefore, the aim of the present study was to explore the protective effect of BMSCs in rats with SCI. A total of 45 adult male Sprague-Dawley rats were randomly divided into three groups; the SCI group (n=15), the BMSC group (n=15) and the sham-operation group (n=15). In the SCI and BMSC treatment groups, a modified Allen’s weight-drop technique was used to induce SCI. The BMSC treatment group received an injection of BMSCs using a microneedle into the epicenter of the spinal cord 24 h after injury. Rats in the sham-operation group were not subjected to SCI; however, the corresponding vertebral laminae were removed. Seven days after transplantation, a rapid recovery was observed in the Basso, Beattie and Bresnahan (BBB) scores of the BMSC treatment group, whereas the BBB scores in the SCI group remained low (P<0.05). Caspase-12 expression in the SCI group was increased compared with that in the sham-operation group, whereas caspase-12 expression was attenuated 24 h after transplantation in the BMSC treatment group (P<0.05). In conclusion, the transplantation of BMSCs may improve locomotor function and attenuate caspase-12 expression following SCI. Therefore, it is likely to be an effective strategy for preventing severe injury of the spinal cord.
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影响因子：
4
作者：
Quiroz-Baez, Ricardo;Ferrera, Patricia;Arias, Clorinda
通讯作者：
Arias, Clorinda
影响因子：
7.2
作者：
Martinez, Juan A.;Zhang, Zhiqun;Larner, Stephen F.
通讯作者：
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影响因子：
4.2
作者：
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通讯作者：
Fehlings, MG
影响因子：
6.2
作者：
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通讯作者：
Whittemore, Scott R.
影响因子：
4.8
作者：
Chiba, Yasuhiro;Kuroda, Satoshi;Iwasaki, Yoshinobu
通讯作者：
Iwasaki, Yoshinobu