Identification of ABCA5 among ATP-Binding Cassette Transporter Family as a New Biomarker for Colorectal Cancer.
Identification of ABCA5 among ATP-Binding Cassette Transporter Family as a New Biomarker for Colorectal Cancer.
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DOI：
10.1155/2022/3399311
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发表时间：
2022
影响因子：
--
通讯作者：
Qin, Changjiang
中科院分区：
文献类型：
--
作者：
Bu, Peilong;Xiao, Yafei;Hu, Shaowen;Jiang, Xiaowei;Tan, Cong;Qiu, Mengyuan;Huang, Wanting;Li, Mengmeng;Li, Quanying;Qin, Changjiang
结直肠癌（CRC）的发病率和死亡率不断上升，迫切需要更新的生物标志物。ABC转运蛋白家族是一个广泛存在的膜结合蛋白家族，参与转运与ATP水解相关的底物，包括代谢物、氨基酸、肽和蛋白质、甾醇和脂质、有机和无机离子、糖、金属和药物。它们在维持体内平衡中起着重要作用。本研究旨在通过Cancer Genome Atlas （TCGA）和GEO （gene Expression Omnibus）数据集的数据挖掘，寻找ABC转运基因家族中用于诊断和预后的新标记。共纳入980个样本，包括来自5个不同数据集的684例CRC患者和296例对照。在STRING数据库和DAVID数据库中分别构建PPI （protein-protein interaction）网络和通路分析。此外，还使用GSEA（基因集富集分析）和WGCNA（加权基因共表达网络分析）进行功能分析。经过多轮筛选和验证，49个基因中仅保留ABCB5基因。结果表明，ABCA5在结直肠癌中表达降低，且ABCA5高表达的患者OS较好，可为今后更好地管理和治疗结直肠癌提供指导。
The increasing incidence and mortality of colorectal cancer (CRC) urgently requires updated biomarkers. The ABC transporter family is a widespread family of membrane-bound proteins involved in the transportation of substrates associated with ATP hydrolysis, including metabolites, amino acids, peptides and proteins, sterols and lipids, organic and inorganic ions, sugars, metals, and drugs. They play an important role in the maintenance of homeostasis in the body. This study aims to search for new markers in the ABC transporter gene family for diagnostic and prognostic purposes through data mining of The Cancer Genome Atlas (TCGA) and GEO (Gene Expression Omnibus) datasets. A total of 980 samples, including 684 CRC patients and 296 controls from five different datasets, were included for analysis. The construction of the PPI (protein-protein interaction) network and pathway analysis were performed in STRING database and DAVID (database for annotation, visualization, and integrated discovery), respectively. In addition, GSEA (gene set enrichment analysis) and WGCNA (weighted gene co-expression network analysis) were also used for functional analysis. After several rounds of screening and validation, only the ABCB5 gene was retained among the 49 genes. The results demonstrated that ABCA5 expression is reduced in CRC and patients with high ABCA5 expression have better OS, which can provide guidance for better management and treatment of CRC in the future.
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DOI：
10.4161/cc.21476
发表时间：
2012-08-15
期刊：
Cell cycle (Georgetown, Tex.)
影响因子：
--
作者：
Alla V;Kowtharapu BS;Engelmann D;Emmrich S;Schmitz U;Steder M;Pützer BM
通讯作者：
Pützer BM
影响因子：
4.5
作者：
DeStefano GM;Kurban M;Anyane-Yeboa K;Dall'Armi C;Di Paolo G;Feenstra H;Silverberg N;Rohena L;López-Cepeda LD;Jobanputra V;Fantauzzo KA;Kiuru M;Tadin-Strapps M;Sobrino A;Vitebsky A;Warburton D;Levy B;Salas-Alanis JC;Christiano AM
通讯作者：
Christiano AM
影响因子：
2.8
作者：
Duz, Mehmet Bugrahan;Karatas, Omer Faruk
通讯作者：
Karatas, Omer Faruk
影响因子：
--
作者：
Guo, Ya;Wang, Zhong Wei;Wang, Ya Li
通讯作者：
Wang, Ya Li
影响因子：
10.3
作者：
Hedditch, Ellen L.;Gao, Bo;Henderson, Michelle J.
通讯作者：
Henderson, Michelle J.