Folic acid-conjugated core/shell ZnS:Mn/ZnS quantum dots as targeted probes for two photon fluorescence imaging of cancer cells
Folic acid-conjugated core/shell ZnS:Mn/ZnS quantum dots as targeted probes for two photon fluorescence imaging of cancer cells
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DOI：
10.1016/j.actbio.2010.10.012
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发表时间：
2011-03-01
影响因子：
9.7
通讯作者：
Schneider, Raphael
中科院分区：
文献类型：
--
作者：
Geszke, Malgorzata;Murias, Marek;Schneider, Raphael
本工作提出了一种制备水溶性锰掺杂核壳型ZnS/ZnS量子点（ZnS：Mn/ZnS）的新方法。通过成核掺杂策略制备Mn掺杂的ZnS核，并且通过在100 ℃下分解Zn 2 +-3-巯基丙酸（MPA）络合物在ZnS：Mn d-点上生长ZnS壳。发现当Mn ~（2+）掺杂量为4.0at.%时，壳层生长后，在590 nm附近的Mn ~（2+）T-4（1）->（6）A（1）荧光发射显著增强。核/壳纳米晶体的光致发光量子产率类似于22%。通过透射电子显微镜对纳米颗粒的结构和组成进行了表征。X射线衍射、X射线光电子能谱和动态光散射。表面MPA分子有利于ZnS：Mn/ZnS量子点在水介质中的分散，并使其与靶向叶酸分子的缀合成为可能。叶酸受体介导的叶酸共轭的ZnS：Mn/ZnS量子点的交付证明使用共聚焦显微镜与双光子激发。裸QD和叶酸缀合QD在孵育72小时后在高浓度（mmol范围）下仅对叶酸受体阳性T47 D癌细胞和MCF-7细胞（用作参考）表现出弱的细胞毒性。(C)2010 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
This work presents a novel approach to producing water soluble manganese-doped core/shell ZnS/ZnS quantum dots (ZnS:Mn/ZnS). The Mn-doped ZnS core was prepared through a nucleation doping strategy and a ZnS shell was grown on ZnS:Mn d-dots by decomposition of Zn2+-3-mercaptopropionic acid (MPA) complexes at 100 degrees C. It was found that the Mn2+ T-4(1) -> (6)A(1) fluorescence emission at similar to 590 nm significantly increased after growth of the shell when the Mn2+ doping content was 4.0 at.%. A photoluminescence quantum yield of similar to 22% was obtained for core/shell nanocrystals. The nanoparticles were structurally and compositionally characterized by transmission electron microscopy. X-ray diffraction, X-ray photoelectron spectroscopy, and dynamic light scattering. The surface MPA molecules favor the dispersion of ZnS:Mn/ZnS QDs in aqueous media and make possible conjugation with targeting folic acid molecules. The folate receptor-mediated delivery of folic acid-conjugated ZnS:Mn/ZnS QDs was demonstrated using confocal microscopy with biphotonic excitation. Bare and folate-conjugated QDs exhibit only weak cytotoxicity towards folate receptor-positive T47D cancer cells and MCF-7 cells, used as a reference, at high concentrations (mmolar range) after 72 h incubation. (C) 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.