Restoration by ribosomal protein S1 of the defective translation in a temperature-sensitive mutant of Escherichia coli K-12: characterization and genetic studies
Restoration by ribosomal protein S1 of the defective translation in a temperature-sensitive mutant of Escherichia coli K-12: characterization and genetic studies
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通过核糖体蛋白 S1 修复大肠杆菌 K-12 温度敏感突变体中的缺陷翻译：表征和遗传学研究
DOI：
10.1128/jb.127.3.1157-1166.1976
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发表时间：
1976
影响因子：
3.2
通讯作者：
B. Maruo
中科院分区：
文献类型：
--
作者：
H. Ohsawa;B. Maruo
分离到一株对温度敏感的大肠杆菌突变株，该突变株在体外翻译大肠杆菌内源信使核糖核酸所需的核糖体洗涤蛋白(S)中存在温敏缺陷。结果发现，30S核糖体蛋白S1挽救了突变体核糖体洗涤蛋白(S)的缺陷，当7S核糖体中加入1mol蛋白S1时，可完全恢复到野生型水平。导致这种缺陷的突变TSS在21min被定位，并与pyrD基因紧密连锁，该基因的区域与编码许多核糖体蛋白的其他基因的区域完全不同。这些结果表明，该突变所指定的基因与30S核糖体蛋白S1的功能有关。
A temperature-sensitive mutant of Escherichia coli was isolated that had a temperature-sensitive defect in ribosomal-wash protein(s) required for translation in vitro of E. coli endogenous messenger ribonucleic acid. It was found that 30S ribosomal protein S1 rescued the defect in the ribosomal-wash protein(s) of the mutant and that the complete restoration to the wild-type level was attained when 1 mol of protein S1 was added to 1 mol of 70S ribosome. The mutation, tss, causing such a defect was mapped at 21 min and was closely linked to the pyrD locus, the region of which was entirely different from that of the other genes coding for the many ribosomal proteins of E. coli. These results indicate that the gene specified by this mutation is involved in the function of the 30S ribosomal protein S1.