Low-density cultures of bovine chondrocytes: effects of scaffold material and culture system
Low-density cultures of bovine chondrocytes: effects of scaffold material and culture system
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DOI：
10.1016/j.biomaterials.2004.06.038
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发表时间：
2005-05-01
期刊：
影响因子：
14
通讯作者：
Athanasiou, KA
中科院分区：
文献类型：
--
作者：
Hu, JC;Athanasiou, KA
将软骨细胞以 1000 万个细胞/ml 支架体积接种在琼脂糖或聚乙醇酸 (PGA) 无纺网上，以评估这两种生物材料对旋转壁生物反应器中软骨细胞低密度培养的影响。对于静态培养和生物反应器培养，琼脂糖构建体比其 PGA 对应物含有更多的糖胺聚糖。然而。与琼脂糖相比，在两种培养条件下，PGA 构建体都含有更多的胶原蛋白。对于本研究的丝束接种密度，在比较 S 周后的胶原含量时，在生物反应器中培养的 PGA 构建体并未优于静态培养物。 PGA 结构的机械性能也没有随着培养时间的延长而提高。用琼脂糖培养物观察到类似的结果。尽管静态培养和生物反应器培养琼脂糖构建体在培养期结束时都表现出聚集模量的增加。与 PGA 培养一样，在琼脂糖中培养的软骨细胞可能需要更高的密度才能从生物反应器环境中获益。 (C) 2004 Elsevier Ltd. 保留所有权利。
Chondrocytes were seeded on either agarose or polyglycolic acid (PGA) unwoven meshes at 10 million cells, ml of scaffold volume to evaluate the effect that these two biomaterials have on the low-density culture of chondrocytes in a rotating-wall bioreactor. For both static and bioreactor Culture, agarose constructs contained more glycosaminoglycan than their PGA counterparts. However. the PGA constructs contained more collagen for both culture conditions when compared to agarose. For the tow seeding density of this study, PGA Constructs cultured in the bioreactor did not outperform static cultures when comparing collagen content after S weeks. The mechanical properties of the PGA Constructs also did not improve with culture time. Similar results were observed with the agarose culture. though both static- and bioreactor-culture agarose constructs exhibited increases in aggregate modulus at the end of the culture period. As in PGA culture, chondrocytes cultured in agarose may require a higher density to reap the benefits of the bioreactor environment. (C) 2004 Elsevier Ltd. All rights reserved.