Biochemical evidence for presence of dopamine beta-hydroxylase in rat retina.
Biochemical evidence for presence of dopamine beta-hydroxylase in rat retina.
复制标题
大鼠视网膜中存在多巴胺β-羟化酶的生化证据。
DOI：
10.1016/0006-8993(88)90380-0
复制
发表时间：
1988
期刊：
影响因子：
2.9
通讯作者：
Ruggiero,DA
中科院分区：
文献类型：
--
作者：
Park,DH;Joh,TH;Anwar,M;Ruggiero,DA
以前，我们用免疫细胞化学方法证明，大鼠视网膜内核层中的某些苯乙醇胺N-甲基转移酶神经元含有其他儿茶酚胺合成酶，包括酪氨酸羟化酶和芳香族-1-氨基酸脱羧酶，但不含去甲肾上腺素生物合成酶多巴胺β-羟化酶（DBH）。在本研究中，通过使用灵敏的DBH放射酶测定，我们证明了在视网膜提取物中存在DBH酶活性。然而，免疫细胞化学研究未能证明DBH免疫反应性周核，即使在用轴突运输抑制剂秋水仙素预处理的动物中也是如此。这些差异的调查结果的可能原因进行了讨论。
Previously, we demonstrated immunocytochemically that certain phenylethanolamineN-methyltransferase neurons in the inner nuclear layer of rat retina contain other catecholamine synthesizing enzymes, including tyrosine hydroxylase and aromatic-l-amino acid decar☐ylase but not dopamine β-hydroxylase (DBH), the norepinephrine biosynthetic enzyme. In the present study by using a sensitive radioenzymatic assay for DBH we demonstrated the presence of DBH enzymatic activity in retinal extracts. Immunocytochemical studies, however, failed to demonstrate DBH-immunoreactive perikarya even in animals pretreated with colchicine, an inhibitor of axonal transport. Probable causes for these discrepant findings are discussed.