Rapid propagation of membrane tension at retinal bipolar neuron presynaptic terminals.
Rapid propagation of membrane tension at retinal bipolar neuron presynaptic terminals.
复制标题
视网膜双极神经元突触前末梢膜张力的快速传播。
DOI：
10.1126/sciadv.abl4411
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发表时间：
2022
期刊：
影响因子：
13.6
通讯作者：
Karatekin,Erdem
中科院分区：
文献类型：
--
作者：
GomisPerez,Carolina;Dudzinski,NatashaR;Rouches,Mason;Landajuela,Ane;Machta,Benjamin;Zenisek,David;Karatekin,Erdem
许多细胞活动，例如细胞迁移、细胞分裂、吞噬作用和胞吐作用，都会产生膜张力梯度并受到膜张力梯度的调节。膜张力梯度驱动膜流，但质膜流放松张力梯度的速度存在争议。在这里，我们证明膜张力可以快速或缓慢地传播，跨越速度的数量级，具体取决于细胞类型。在专门负责快速突触小泡周转的神经元末梢中，膜张力在几秒钟内达到平衡。相比之下，膜张力不会在分泌儿茶酚胺的神经内分泌肾上腺嗜铬细胞中传播。胞吐作用的刺激导致突触末端膜张力迅速全面降低，但由于胞吞作用而缓慢恢复。因此，膜流动和张力平衡可以适应不同的膜回收要求。
Many cellular activities, such as cell migration, cell division, phagocytosis, and exo-endocytosis, generate and are regulated by membrane tension gradients. Membrane tension gradients drive membrane flows, but there is controversy over how rapidly plasma membrane flow can relax tension gradients. Here, we show that membrane tension can propagate rapidly or slowly, spanning orders of magnitude in speed, depending on the cell type. In a neuronal terminal specialized for rapid synaptic vesicle turnover, membrane tension equilibrates within seconds. By contrast, membrane tension does not propagate in neuroendocrine adrenal chromaffin cells secreting catecholamines. Stimulation of exocytosis causes a rapid, global decrease in the synaptic terminal membrane tension, which recovers slowly due to endocytosis. Thus, membrane flow and tension equilibration may be adapted to distinct membrane recycling requirements.