Gait analysis detects early changes in transgenic SOD1(G93A) mice
Gait analysis detects early changes in transgenic SOD1(G93A) mice
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DOI：
10.1002/mus.20228
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发表时间：
2005-07-01
期刊：
影响因子：
3.4
通讯作者：
Seburn, KL
中科院分区：
文献类型：
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作者：
Wooley, CM;Sher, RB;Seburn, KL
运动神经元疾病的有效治疗或治愈需要了解不可逆细胞损失之前的疾病过程。为了研究这些早期阶段并评估相关动物模型中的潜在治疗方法，需要进行灵敏的功能测定。为此，我们试图确定 SOD1 转基因小鼠的步态模式是否在出现明显症状之前发生变化。我们使用简化的基于视频的方法比较了 8 周龄和 10 周龄时 C57BL/6J 和 B6.SOD 1 转基因小鼠的跑步机步态。 B6.SOD1 小鼠的步幅和站立时间明显比对照组长 8 周。与疾病进展一致，B6.SOD1 小鼠的后爪测量值显示出比前爪更大的变化。对照小鼠和 B6.SOD1 小鼠之间的差异在第 10 周时增加，但这只是因为重复测试比 B6.SOD1 小鼠更能引起对照小鼠的习惯。这些结果共同表明，简化的步态分析对小鼠运动系统疾病的早期过程敏感。
The effective treatment or cure of motoneuron disease will require understanding the disease processes that precede irreversible cell loss. To study these early stages, and to evaluate potential treatments in relevant animal models, requires a sensitive functional assay. To this end, we sought to determine whether the gait pattern of SOD1 transgenic mice changed prior to overt symptoms. Using a simplified video-based approach we compared the treadmill gait of C57BL/6J and B6.SOD 1 transgenic mice at 8 and 10 weeks of age. B6.SOD1 mice had significantly longer stride and stance times than controls by 8 weeks. Consistent with disease progression, hindpaw measures of B6.SOD1 mice showed larger changes than front paws. Differences between control and B6.SOD1 mice increased at 10 weeks, but only because repeat testing caused habituation in control mice to a greater extent than in B6.SOD1 mice. Together the results demonstrate that simplified gait analysis is sensitive to early processes of motor system disease in mice.