Expression of glial fibrillary acidic protein, vimentin, fibronectin, and N-myc oncoprotein in primary human brain tumor cell explants.
Expression of glial fibrillary acidic protein, vimentin, fibronectin, and N-myc oncoprotein in primary human brain tumor cell explants.
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原代人脑肿瘤细胞外植体中胶质纤维酸性蛋白、波形蛋白、纤连蛋白和 N-myc 癌蛋白的表达。
DOI：
10.1159/000120590
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发表时间：
1991
影响因子：
0.7
通讯作者：
Galloway,PG
中科院分区：
文献类型：
--
作者：
Mapstone,TB;Galloway,PG
研究了23例人胶质瘤（7例星形细胞瘤、11例间变性星形细胞瘤、3例室管膜瘤和2例髓母细胞瘤）的细胞形态和胶质纤维酸性蛋白（GFAP）、波形蛋白、纤维连接蛋白和N-myc癌蛋白的表达。最常见的抗原表型包括gap阳性和纤维连接蛋白阳性的细胞。低分级和高分级星形细胞瘤在体外多次传代后均保留GFAP的表达。体外神经胶质肿瘤的建立不一定会导致GFAP在传代早期表达缺失，GFAP和纤维连接蛋白的表达也不是相互排斥的。N-myc癌蛋白仅见于两个标本，一个间变性星形细胞瘤和一个室管膜瘤。
Cultured cells of explants from 23 human gliomas (seven astrocytomas, eleven anaplastic astrocytomas, three ependymal tumors and two medulloblastomas) were studied to examine cell morphology and expression of glial fibrillary acidic protein (GFAP), vimentin, fibronectin, and N-myc oncoprotein. The most common antigenic phenotype consisted of cells that were GFAP-positive and fibronectin-positive. Both low and high grade astrocytomas retained GFAP expression after several passages in vitro. The establishment of glial tumors in vitro may not necessarily result in loss of GFAP expression early in passage nor is expression of GFAP and fibronectin mutually exclusive. N-myc oncoprotein was seen in only two specimens, one anaplastic astrocytoma and one ependymoma.