Effects of glucose, insulin, glucagon, LPS and poly (I:C) on adiponectin receptors expression in grass carp (Ctenophcuyngodon idellus)
Effects of glucose, insulin, glucagon, LPS and poly (I:C) on adiponectin receptors expression in grass carp (Ctenophcuyngodon idellus)
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葡萄糖、胰岛素、胰高血糖素、脂多糖和聚(I:C)对草鱼脂联素受体表达的影响
DOI：
10.1016/j.aquaculture.2020.735501
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发表时间：
2020
期刊：
影响因子：
4.5
通讯作者：
Nie Guoxing
中科院分区：
文献类型：
--
作者：
Qin Chaobin;Zhao Wenli;Deng Dapeng;Lu Ronghua;Yang Guokun;Yan Xiao;Meng Xiaolin;Nie Guoxing
脂联素通过与受体（AdipoR 1和AdipoR 2）结合在哺乳动物的能量代谢和免疫中发挥重要作用。然而，它们在鱼类中还没有被完全理解。研究了脂联素受体（AdipoRs）mRNA在草鱼体内的分布，以及在葡萄糖负荷、胰岛素或胰高血糖素刺激、脂多糖（LPS）和聚肌胞苷酸（poly（I：C））刺激下的表达变化。在葡萄糖负荷方面，以1.67mg/g体重的葡萄糖分别灌胃给鱼1、3、6、12 h。对于胰岛素或胰高血糖素刺激，草鱼原代肝细胞与0、10、100、1000 nM胰岛素或胰高血糖素孵育1、3、6、12 h。免疫攻击实验中，草鱼腹腔注射LPS或poly（I：C）（5 μg/g BW），分别处理3、6、12、24、48 h。最后采用实时荧光定量PCR检测各组细胞AdipoRs mRNA的表达。结果表明，AdipoRs mRNA在草鱼各组织中广泛表达。adipoR 1amRNA在脑组织中高表达，而adipoR 2 mRNA在脂肪组织中表达最高。葡萄糖负荷在给药后6 h上调肝脏中adipoR 1和adipoR 2 mRNA的表达，3 h降低肌肉中adipoR 1a的表达。胰岛素在1-12 h显著增强肝细胞adipoR 1和adipoR 2 mRNA的表达，并在1、3和12 h促进adipoR 1 amRNA的表达，而胰高血糖素对其表达影响不大。LPS和poly（I：C）刺激后6 h和12 h，肝脏中AdipoRs表达增加，肌肉中AdipoRs表达略有下降或保持不变。另外，LPS可促进肾脏和脾脏中AdipoRs的表达，而poly（I：C）仅诱导肾脏中adipoR 1amRNA的表达，并增加脾脏中adipoR 2 mRNA的表达。这些结果提示，AdipoRs可能是草鱼胰岛素和免疫应答的靶点之一，但是否为直接靶点还有待进一步研究。
Adiponectin plays critical roles in energy metabolism and immunity of mammals by binding to its receptors (AdipoR1 and AdipoR2). However, they have not been fully understood in fish. The present study examined the distribution of adiponectin receptors (AdipoRs) mRNA in grass carp, and the changes of their expressions under glucose load, insulin or glucagon stimulation, lipopolysaccharide (LPS) or polyinosinic:polycytidylic acid [poly (I:C)] challenge. As for glucose load, fish were orally administrated with glucose (1.67 mg/g BW) for 1, 3, 6, 12 h. For insulin or glucagon stimulation, the primary hepatocytes of grass carp were incubated with 0, 10, 100, 1000 nM insulin or glucagon for 1, 3, 6, 12 h. In the immune challenge experiment, grass carp were intraperitoneally injected with LPS or poly (I:C) (5 μg/g BW) for 3, 6, 12, 24, 48 h. Finally, AdipoRs mRNA expression in all experiments was detected by Real-time quantitative PCR. The results showed that AdipoRs mRNA was widely expressed in various tissues of grass carp. TheadipoR1amRNA was highly expressed in the brain regions, and the highest level ofadipoR2mRNA was observed in the adipose tissue. Glucose load up-regulatedadipoR1bandadipoR2mRNA expressions in the liver at 6 h post administration and decreasedadipoR1aexpression in the muscle at 3 h. Insulin significantly enhancedadipoR1bandadipoR2mRNA expressions in the hepatocytes at 1–12 h, and promotedadipoR1amRNA expression at 1, 3 and 12 h,while glucagon had little effect on their expressions. LPS and poly (I:C) stimuli induced AdipoRs expression in the liver at 6 and 12 h, and their expressions in the muscle were decreased slightly or remained unchanged. In addition, LPS promoted AdipoRs expression in the kidney and spleen, whereas poly (I:C) only inducedadipoR1amRNA expression in the kidney, and increasedadipoR2mRNA level in the spleen. These results suggest that AdipoRs may act as one of the targets of insulin and immune response in grass carp, but whether they are direct target remains to be further studied.