Soleus, plantaris and gastrocnemius VEGF mRNA responses to hypoxia and exercise are preserved in aged compared with young female C57BL/6 mice
Soleus, plantaris and gastrocnemius VEGF mRNA responses to hypoxia and exercise are preserved in aged compared with young female C57BL/6 mice
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与年轻雌性 C57BL/6 小鼠相比，老龄小鼠的足底、足底和腓肠肌 VEGF mRNA 对缺氧和运动的反应保持不变
DOI：
10.1111/j.1748-1716.2006.01609.x
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发表时间：
2006-10-01
影响因子：
6.3
通讯作者：
Zwetsloot, K. A.
中科院分区：
文献类型：
--
作者：
Gavin, T. P.;Westerkamp, L. M.;Zwetsloot, K. A.
目的：在人类中，骨骼肌毛细血管化和血管内皮生长因子（VEGF）mRNA对急性运动的反应在老年人比年轻人低。运动诱导的VEGF mRNA的增加已被提出涉及VEGF的低氧调节，并且被认为是纤维类型依赖性的。我们假设，衰减VEGF mRNA的反应，缺氧和运动与先进的年龄将是最大的氧化与糖酵解muscle.Methods：3-和24个月大的女性C57 BL/ 6小鼠暴露于急性缺氧（FIO 2 = 0.06）或进行一个单一的运动（65%的最大跑步机运行速度）回合。结果：老年人比目鱼肌、跖肌和腓肠肌中VEGF mRNA表达量均高于青年人，而急性缺氧或急性运动对VEGF mRNA表达的影响与老年人相似。形态学分析显示，老年人足底IIA型纤维百分比和IIB型毛细血管化程度高于青年人，腓肠肌IIB型纤维横截面积（FCSA）低于青年人。这些发现表明，衰老不会损害小鼠非病理性血管生成的潜力，并且急性运动会增加比目鱼肌、跖肌和腓肠肌中的VEGF mRNA，它们在纤维类型百分比上差别很大。
Aims: In humans, skeletal muscle capillarization and the vascular endothelial growth factor (VEGF) mRNA response to acute exercise are lower in aged compared with young. The exercise-induced increase in VEGF mRNA has been proposed to involve hypoxic regulation of VEGF and is believed to be fibre type-dependent. We hypothesized that attenuated VEGF mRNA responses to hypoxia and exercise with advanced age would be greatest in oxidative vs. glycolytic muscles.Methods: 3- and 24-month-old female C57BL/ 6 mice were exposed to acute hypoxia (FIO2 = 0.06) or performed a single exercise (65% of maximum treadmill running speed) bout. Capillarization and VEGF mRNA were analysed in the soleus, plantaris and gastrocnemius muscles.Results: In each muscle, VEGF mRNA was greater in aged compared with young, while the VEGF mRNA response to acute hypoxia or acute exercise was similar between young and aged. Morphological analysis revealed that type IIA fibre percentage and type IIB capillarization in the plantaris were greater and type IIB fibre cross-sectional area (FCSA) in the gastrocnemius was smaller in aged compared with young.Conclusions: These findings suggest that ageing does not impair the potential for non-pathological angiogenesis in mice and that acute exercise increases VEGF mRNA in the soleus, plantaris and gastrocnemius muscles, which differ considerably in fibre type percentage.