DNA ligase I mRNA and enzyme levels in human hematopoietic cells under dimethyl sulfoxide-induced growth-arrest and differentiation.
DNA ligase I mRNA and enzyme levels in human hematopoietic cells under dimethyl sulfoxide-induced growth-arrest and differentiation.
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二甲基亚砜诱导的生长停滞和分化下人类造血细胞中 DNA 连接酶 I mRNA 和酶水平。
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
K. Tsukada
中科院分区：
文献类型：
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作者：
H. Teraoka;M. Sawai;K. Yamamoto;K. Tsukada
经1.5%二甲基亚砜(DMSO)处理4天后，细胞周期中DNA连接酶I的活性水平仍保持在G1期细胞的一半左右，而在终末分化的HL-60细胞中，DNA连接酶I的活性仅为活跃生长的HL-60细胞的1/3。相反，DNA连接酶I的mRNA在G1期阻滞的Raji和分化的HL-60细胞中几乎可以忽略不计。在被DMSO抑制的Raji细胞中，DNA连接酶I在释放后9h开始稳定表达，18h达到高峰，提示DNA连接酶I的基因表达与细胞增殖活性密切相关，而与该酶的活性水平无关。
About half the activity level of DNA ligase I in cycling human lymphoblastoid cells (Raji and Akata) remained in the cells arrested at G1 by a 4-day treatment with 1.5% dimethyl sulfoxide (DMSO), and one-third the enzyme activity in actively growing promyelocytic leukemia cells HL-60 was detected in the terminally differentiated cells after DMSO-treatment. In contrast, DNA ligase I mRNA was negligible in the G1-arrested Raji and differentiated HL-60 cells. The steady-state mRNA level was increased 9 h after release from DMSO in the G1-arrested Raji cells and reached a maximum at 18 h. These results indicate that gene expression of human DNA ligase I, but not activity level of the enzyme, is closely correlated with activity of cell proliferation.