Intracellular Staphylococcus aureus -: A mechanism for the indolence of osteomyelitis
Intracellular Staphylococcus aureus -: A mechanism for the indolence of osteomyelitis
复制标题
DOI：
10.1302/0301-620x.85b6.13509
复制
发表时间：
2003-08-01
影响因子：
--
通讯作者：
Hudson, M
中科院分区：
文献类型：
--
作者：
Ellington, JK;Harris, M;Hudson, M
金黄色葡萄球菌是导致约80%的人类骨髓炎病例的细菌病原体。它可以侵入并停留在成骨细胞内。细胞内葡萄球菌的命运。我们将人成骨细胞暴露于葡萄球菌。金黄色。感染后，将成骨细胞用Triton X-100裂解或胰蛋白酶消化。培养并计数从胰蛋白酶消化和裂解的成骨细胞释放的细菌。然后将回收的细菌菌落引入人成骨细胞的另外的培养物中。从两种技术回收的金黄色葡萄球菌是等效的。葡萄球菌金黄色葡萄球菌从时间0和感染后24小时恢复，随后进行裂解/胰蛋白酶消化，能够侵入人成骨细胞的第二次培养。金黄色葡萄球菌和葡萄球菌从垂死或死亡的成骨细胞释放的金黄色葡萄球菌能够再感染培养中的人成骨细胞。
Staphylococcus aureus is the bacterial pathogen which is responsible for approximately 80% of all cases of human osteomyelitis. It can invade and remain within osteoblasts. The fate of intracellular Staph. aureus after the death of the osteoblast has not been documented.We exposed human osteoblasts to Staph. aureus. After infection, the osteoblasts were either lysed with Triton X-100 or trypsinised. The bacteria released from both the trypsinised and lysed osteoblasts were cultured and counted. Colonies of the recovered bacteria were then introduced to additional cultures of human osteoblasts.The number of intracellular Staph. aureus recovered from the two techniques was equivalent. Staph. aureus recovered from time zero and 24 hours after infection, followed by lysis/trypsinisation, were capable of invading a second culture of human osteoblasts.Our findings indicate that dead or dying osteoblasts are capable of releasing viable Staph. aureus and that Staph. aureus released from dying or dead osteoblasts is capable of reinfecting human osteoblasts in culture.