Characterization and comparison of the bacterial microbiota in different gastrointestinal tract compartments in horses
Characterization and comparison of the bacterial microbiota in different gastrointestinal tract compartments in horses
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DOI：
10.1016/j.tvjl.2015.03.018
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发表时间：
2015-07-01
影响因子：
2.2
通讯作者：
Weese, J. S.
中科院分区：
文献类型：
--
作者：
Costa, M. C.;Silva, G.;Weese, J. S.
新测序技术的进步使复杂的微生物群落（包括肠道微生物群落）得到了更全面的表征。极端环境过滤器的存在，如低pH值，消化酶和厌氧条件沿着管道，作用于每个隔室中独特细菌的选择。肠道微生物群对维持健康有着巨大的影响。然而，关于马的不同肠道隔室中存在的细菌的数据很少。在这项研究中，高通量测序被用来表征和比较细菌的配置文件从不同的肠腔室的11匹马计划安乐死的原因以外的胃肠问题，即使在高分类水平的车厢之间的显着差异被发现，厚壁菌门构成的主要细菌门在所有的车厢。乳杆菌属和八叠球菌属（Sarcina spp.）占主导地位的胃和链球菌属的显着增加。发生在十二指肠。放线杆菌和狭义梭菌是回肠中最丰富的属，而来自疣状微菌亚门5纲的“5属未定菌”是通过粪便从大结肠中最丰富的属。有一个显着增加的多样性向远端肠道与类似的配置文件中观察到的盲肠通过粪便在类的水平。组成马肠道的细菌群体在隔室之间变化很大，粪便样品可用作远端隔室中发生的变化的代表。(C)2015爱思唯尔有限公司版权所有。
The advance of new sequencing technologies has allowed more comprehensive characterization of complex microbial communities, including the ones inhabiting the intestinal tract. The presence of extreme environmental filters, such as low pH, digestive enzymes and anaerobic conditions along the tract, acts on the selection of unique bacteria in each compartment. The intestinal microbiota has an enormous impact on the maintenance of health. However, data about the bacteria present in the different intestinal compartments of horses are sparse. In this study, high throughput sequencing was used to characterize and compare bacterial profiles from different intestinal compartments of 11 horses scheduled for euthanasia for reasons other than gastrointestinal problems.Marked differences among compartments even at high taxonomic levels were found, with Firmicutes comprising the main bacterial phylum in all compartments. Lactobacillus spp. and Sarcina spp. predominated in the stomach and a marked increase of Streptococcus spp. occurred in the duodenum. Actinobacillus and Clostridium sensu stricto were the most abundant genera in the ileum and '5 genus incertae sedis', a genus from the Subdivision 5 class of the Verrucomicrobia, was the most abundant from the large colon through feces. There was a significant increase in diversity towards the distal gut with similar profiles observed from the cecum through feces at the class level. The bacterial population comprising the equine intestinal tract varies greatly among compartments and fecal samples may be useful as representative of changes occurring in the distal compartments. (C) 2015 Elsevier Ltd. All rights reserved.