Monitoring long-term evolutionary changes following Wolbachia introduction into a novel host: the Wolbachia popcorn infection in Drosophila simulans
Monitoring long-term evolutionary changes following Wolbachia introduction into a novel host: the Wolbachia popcorn infection in Drosophila simulans
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DOI：
10.1098/rspb.2010.0166
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发表时间：
2010-07-07
影响因子：
4.7
通讯作者：
Weeks, Andrew R.
中科院分区：
文献类型：
--
作者：
Carrington, Lauren B.;Hoffmann, Ary A.;Weeks, Andrew R.
沃尔巴克氏菌可作为害虫管理的生防剂；特别是沃尔巴克氏菌的变种wMelPop(爆米花)可缩短宿主寿命，并可能有助于抑制登革热。然而，宿主和沃尔巴克氏菌基因组的长期变化可以改变沃尔巴克氏菌的传播和/或抑制疾病的宿主效应。在这里，我们研究了wMelPop在大约200代前人工感染后对非本地宿主模拟果蝇的表型影响。在爆米花菌株感染的原始I-102遗传背景下，细菌的长期培养和维持在19摄氏度。然后将这些细菌导入到三种大规模繁殖的背景中，并使用四环素创建未受感染的亚系。WMelPop对该物种寿命的影响似乎发生了变化；在一些核背景下，寿命不再在25摄氏度减少，这反映了不同的地理起源、选择或漂移，尽管在30摄氏度的温度下，果蝇寿命的减少仍然很明显。沃尔巴克氏菌影响了生产力和生存能力，以及一些宿主背景下的发育时间。这些发现表明，沃尔巴克氏菌作用的长期减弱可能会损害这种细菌在害虫控制中的有效性。他们还强调了宿主核背景对沃尔巴克氏菌表型效应的重要性。
Wolbachia may act as a biological control agent for pest management; in particular, the Wolbachia variant wMelPop (popcorn) shortens host longevity and may be useful for dengue suppression. However, long-term changes in the host and Wolbachia genomes can alter Wolbachia spread and/or host effects that suppress disease. Here, we investigate the phenotypic effects of wMelPop in a non-native host, Drosophila simulans, following artificial transinfection approximately 200 generations ago. Long-term rearing and maintenance of the bacteria were at 19 degrees C in the original I-102 genetic background that was transinfected with the popcorn strain. The bacteria were then introgressed into three massbred backgrounds, and tetracycline was used to create uninfected sublines. The effect of wMelPop on longevity in this species appears to have changed; longevity was no longer reduced at 25 degrees C in some nuclear backgrounds, reflecting different geographical origin, selection or drift, although the reduction was still evident for flies held at 30 degrees C. Wolbachia influenced productivity and viability, and development time in some host backgrounds. These findings suggest that long-term attenuation of Wolbachia effects may compromise the effectiveness of this bacterium in pest control. They also emphasize the importance of host nuclear background on Wolbachia phenotypic effects.