Fast atom bombardment mass spectrometric quantitative analysis of methionine-enkephalin in human pituitary tissues.

Fast atom bombardment mass spectrometric quantitative analysis of methionine-enkephalin in human pituitary tissues.
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人垂体组织中蛋氨酸脑啡肽的快原子轰击质谱定量分析。

DOI:
10.1021/ac00220a026
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发表时间:
1990
影响因子:
7.4
通讯作者:
Desiderio,DM
Desiderio,DM
中科院分区:
化学1区
文献类型:
--
作者:
Kusmierz,JJ;Sumrada,R;Desiderio,DM

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分别是Y2", Y3", Y4", A4, B3, B4。该产物的离子谱类似于100 ng合成ME的扫描图。我们计算出用于MRM实验的五肽的量对应于定量时探针尖端上总共30 ng (52 pmol)的ME。另一方面,我们估计在获得产物离子谱时,探针尖端上的mei是其3倍,即90 ng (156 pmol)。
Y2", Y3", Y4", A4, B3, and B4, respectively. That product Ion spectrum was similar to a scan of 100 ng of synthetic ME. We calculated that the amount of pentapeptlde for the MRM experiments corresponded to a total of 30 ng (52 pmol) of ME on the probe tip during quantification. On the other hand, we estimated that 3 times more, or 90 ng (156 pmol), MEwas on the probe tip during acquisition of the product ion spectrum.