RpiR homologues may link Staphylococcus aureus RNAIII synthesis and pentose phosphate pathway regulation.
RpiR homologues may link Staphylococcus aureus RNAIII synthesis and pentose phosphate pathway regulation.
复制标题
RpiR 同源物可能将金黄色葡萄球菌 RNAIII 合成和戊糖磷酸途径调节联系起来。
DOI：
10.1128/jb.05930-11
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发表时间：
2011
影响因子：
3.2
通讯作者：
Somerville,GregA
中科院分区：
文献类型：
--
作者：
Zhu,Yefei;Nandakumar,Renu;Sadykov,MaratR;Madayiputhiya,Nandakumar;Luong,ThanhT;Gaupp,Rosmarie;Lee,ChiaY;Somerville,GregA
金黄色葡萄球菌（Staphylococcus aureus）是医学上重要的病原体，其合成广泛的毒力决定因子。许多葡萄球菌毒力决定因子的合成部分受应力诱导的三羧酸（TCA）循环活性变化的调节。与TCA循环应激相关的一种代谢变化是核糖浓度增加，这使我们假设戊糖磷酸途径（PPP）响应调节剂介导了一些TCA循环依赖性调节作用。使用生物信息学，我们确定了三个潜在的核糖响应调节器，属于RpiR家族的转录调节器。为了确定这些RpiR同源物是否影响PPP活性和毒力决定簇合成，将therpiR同源物灭活，并评估对PPP活性和毒力因子合成的影响。三个同源物中的两个（RpiRB和RpiRC）正影响PPP基因rpiAandzwf的转录，而第三个同源物（RpiRA）与其他同源物轻微拮抗。此外，RpiRC的失活改变了RNA III的时间转录，RNA III是群体感应系统的效应分子。这些数据证实了中央代谢和毒力决定簇合成的密切联系，他们建立了一个群体感应依赖调节RNAIII转录的代谢覆盖。
Staphylococcus aureus is a medically important pathogen that synthesizes a wide range of virulence determinants. The synthesis of many staphylococcal virulence determinants is regulated in part by stress-induced changes in the activity of the tricarboxylic acid (TCA) cycle. One metabolic change associated with TCA cycle stress is an increased concentration of ribose, leading us to hypothesize that a pentose phosphate pathway (PPP)-responsive regulator mediates some of the TCA cycle-dependent regulatory effects. Using bioinformatics, we identified three potential ribose-responsive regulators that belong to the RpiR family of transcriptional regulators. To determine whether these RpiR homologues affect PPP activity and virulence determinant synthesis, therpiRhomologues were inactivated, and the effects on PPP activity and virulence factor synthesis were assessed. Two of the three homologues (RpiRB and RpiRC) positively influence the transcription of the PPP genesrpiAandzwf, while the third homologue (RpiRA) is slightly antagonistic to the other homologues. In addition, inactivation of RpiRC altered the temporal transcription of RNAIII, the effector molecule of theagrquorum-sensing system. These data confirm the close linkage of central metabolism and virulence determinant synthesis, and they establish a metabolic override for quorum-sensing-dependent regulation of RNAIII transcription.