Trace determination of 10 β-lactam antibiotics in environmental and food samples by capillary liquid chromatography
Trace determination of 10 β-lactam antibiotics in environmental and food samples by capillary liquid chromatography
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DOI：
10.1016/j.chroma.2009.09.042
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发表时间：
2009-11-20
影响因子：
4.1
通讯作者：
Cruces-Blanco, C.
中科院分区：
文献类型：
--
作者：
Bailon-Perez, M. L.;Garcia-Campana, A. M.;Cruces-Blanco, C.
建立了高效液相色谱-紫外二极管阵列检测器（DAD）检测牛奶、鸡肉和环境水样中10种人用和兽用β-内酰胺类抗生素残留的方法。分析物包括氨苄西林、阿莫西林、青霉素V、青霉素G。氯唑西林、苯唑西林、双氯西林、萘夫西林、哌拉西林和克拉维酸。这些化合物在动物可食用组织中的法律的水平由欧盟理事会法规2377/90规定。对于食品分析，固相萃取（SPE）程序组成的一个串联的绿洲HLB和氧化铝N柱被应用于离线预浓缩和净化。对于水质分析，第一步只是必要的。研究化合物的检测限为0.04-0.06 μ g l（-1）（水样）和0.80-1.40 μ g l（-1）（或μ g kg（-1）（动物源性食品）。不同浓度水平的加标回收率在82.9%~ 98.2%之间，相对标准偏差（RSD）均小于9%。该方法显示了毛细管高效液相色谱法在检测这些广泛应用的抗生素在不同样品中在很低的浓度水平的优势。(C)2009爱思唯尔有限公司版权所有。
A sensitive and reliable method using capillary HPLC with UV-diode array detection (DAD) has been developed and validated for the trace determination of residues of 10 beta-lactam antibiotics of human and veterinary use, in milk, chicken meat and environmental water samples. The analytes included ampicillin, amoxicillin, penicillin V, penicillin G. cloxacillin, oxacillin, dicloxacillin, nafcillin, piperacillin and clavulanic acid. Legal levels are regulated by the EU Council regulation 2377/90 in animal edible tissues for these compounds. For food analysis, a solid-phase extraction (SPE) procedure consisting in a tandem of Oasis HLB and Alumina N cartridges was applied for off-line preconcentration and cleanup. For water analysis, the first step was only necessary. The limits of detection for the Studied compounds were between 0.04-0.06 mu g l(-1) for water samples and 0.80-1.40 mu g l(-1) (or mu g kg(-1)) in the case of foods derived from animals. Average recoveries for fortified samples at different concentration levels ranged between 82.9% and 98.2%, with relative standard deviations (RSDs) lower than 9%. The method showed the advantages of capillary HPLC for the detection of these widely applied antibiotics in different samples at very low concentration levels. (C) 2009 Elsevier B.V. All rights reserved.