An Improved Escherichia Coli Bacterium Detection in Microchannel Based on Dielectrophoresis Impedance Measurements
An Improved Escherichia Coli Bacterium Detection in Microchannel Based on Dielectrophoresis Impedance Measurements
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基于介电泳阻抗测量的改进的微通道中大肠杆菌检测
DOI：
10.33945/sami/ajca.2020.2.3
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
M. Fathollahzadeh
中科院分区：
文献类型：
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作者：
A. Ebrahimi;M. Fathipour;M. Fathollahzadeh
在本研究中，利用 COMSOL Multiphysics 模拟了利用正介电电泳和负介电电泳优点的细菌检测技术。所提出的二维模型具有跨微流体通道的六个微电极。其中四个位于微通道的上侧，其余位于微通道的底侧，分别用作使用负介电电泳的细菌浓缩器和使用正介电电泳的细菌检测器。在该模拟中，目标颗粒是大肠杆菌，它流入微通道并在上侧微电极施加的负介电泳力的作用下被排斥，并被推向位于下游的底侧微电极。最后，通过介电泳阻抗测量方法捕获并检测浓缩的细菌。数值模拟证明，相对于没有负介电泳力，负介电泳力可以提高灵敏度，最终结果表明，80％的释放大肠杆菌被捕获在底侧微电极附近。
In this study, a bacterial detection technique utilizing benefits of both positive and negative dielectrophoresis has been simulated with COMSOL Multiphysics. The two dimensional proposed model has six microelectrodes across microfluidic channel. Four of them were positioned on the upper side and the rest were positioned on the bottom side of the microchannel, which serve as a bacteria concentrator using negative dielectrophoresis and as a bacteria detector using positive dielectrophoresis, respectively. In this simulation, the target particle is Escherichia coli that was flowing into the microchannel and repelled under negative dielectrophoretic force exerted by the upper side microelectrodes, and were pushed toward the bottom side microelectrodes situated at the downstream. Finally, concentrated bacteria have been captured and detected by dielectrophoretic impedance measurement method. The numerical simulation proved that negative dielectrophoretic force could increase sensitivity with respect to the absence of the negative dielectrophoretic force and eventually, results represented that 80 percent of releasing E. coli bacteria trapped near bottom side microelectrodes.