ENVIRONMENT AFFECTS AMINO-ACID PREFERENCE FOR SECONDARY STRUCTURE
ENVIRONMENT AFFECTS AMINO-ACID PREFERENCE FOR SECONDARY STRUCTURE
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DOI：
10.1073/pnas.89.10.4462
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发表时间：
1992-05-15
影响因子：
11.1
通讯作者：
JOHNSON, WC
中科院分区：
文献类型：
--
作者：
ZHONG, LX;JOHNSON, WC
合成了三个模棱两可的氨基酸序列，根据氨基酸偏好预测它们是α螺旋，但从它们各自蛋白质的X射线衍射发现主要是β链。在一些溶剂系统中，我们恢复了由氨基酸偏好预测的α螺旋结构，而在其他系统中，我们模拟蛋白质的内部并产生β链。这些结果是实验证明，环境对于决定氨基酸序列形成的二级结构很重要；因此，仅根据氨基酸序列预测二级结构的方案永远不可能完全成功。
Three equivocal amino acid sequences were synthesized that are predicted to be alpha-helical from amino acid preference but are found to be primarily beta-strand from x-ray diffraction of their respective proteins. In some solvent systems we recover the alpha-helical structure predicted by amino acid preference, whereas in other systems we mimic the interior of the protein and produce a beta-strand. These results are experimental proof that the environment is important in determining the secondary structure formed by an amino acid sequence; therefore schemes that predict secondary structure from amino acid sequence alone can never be totally successful.