A sensitive acetylcholinesterase biosensor based on gold nanorods modified electrode for detection of organophosphate pesticide
A sensitive acetylcholinesterase biosensor based on gold nanorods modified electrode for detection of organophosphate pesticide
复制标题
基于金纳米棒修饰电极的灵敏乙酰胆碱酯酶生物传感器用于检测有机磷农药
DOI：
10.1016/j.talanta.2016.05.002
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发表时间：
2016-08-15
期刊：
影响因子：
6.1
通讯作者：
Liu, Aihua
中科院分区：
文献类型：
--
作者：
Lang, Qiaolin;Han, Lei;Liu, Aihua
研制了一种基于金纳米棒的灵敏的乙酰胆碱酯酶（AChE）生物传感器，用于有机磷农药的检测。与Au@Ag非均相NRs相比，aunr表现出优异的电催化性能，可以在+0.55 V （vs. SCE）下电催化氧化乙酰硫代氯化胆碱（ATCI）水解产物硫代胆碱。利用有机磷农药对AChE活性的抑制作用，制备了AChE/AuNRs/GCE生物传感器。该传感器在1 nM ~ 5 μ M的线性范围内检测对氧磷，在5 nM ~ 1 μ M的线性范围内检测乐果。对氧磷和乐果的检出限分别为0.7 nM和3.9 nM，均低于已有报道的AChE生物传感器。该传感器可恢复95%以上的电流，具有良好的再激活性。此外，该传感器可用于实际水样测量。因此，该传感器具有应用潜力小、灵敏度高、稳定性好的特点，是一种很有前景的农药分析工具。(C) 2016 Elsevier B.V.版权所有
A sensitive amperometric acetylcholinesterase (AChE) biosensor, based on gold nanorods (AuNRs), was developed for the detection of organophosphate pesticide. Compared with Au@Ag heterogeneous NRs, AuNRs exhibited excellent electrocatalytic properties, which can electrocatalytically oxidize thiocholine, the hydrolysate of acetylthiocholine chloride (ATCI) by AChE at +0.55 V (vs. SCE). The AChE/AuNRs/GCE biosensor was fabricated on basis of the inhibition of AChE activity by organophosphate pesticide. The biosensor could detect paraoxon in the linear range from 1 nM to 5 mu M and dimethoate in the linear range from 5 nM to 1 mu M, respectively. The detection limits of paraoxon and dimethoate were 0.7 nM and 3.9 nM, which were lower than the reported AChE biosensor. The proposed biosensor could restore to over 95% of its original current, which demonstrated the good reactivation. Moreover, the biosensor can be applicable to real water sample measurement. Thus, the biosensor exhibited low applied potential, high sensitivity and good stability, providing a promising tool for analysis of pesticides. (C) 2016 Elsevier B.V. All rights reserved.