DNA as a phosphate storage polymer and the alternative advantages of polyploidy for growth or survival.
DNA as a phosphate storage polymer and the alternative advantages of polyploidy for growth or survival.
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DNA作为磷酸盐储存聚合物以及多倍体在生长或存活中的替代优势。
DOI：
10.1371/journal.pone.0094819
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Soppa J
中科院分区：
文献类型：
--
作者：
Zerulla K;Chimileski S;Näther D;Gophna U;Papke RT;Soppa J
Haloferax volcanii使用细胞外DNA作为碳、氮和磷的来源。然而，在没有添加磷的情况下，它也可以生长到有限的程度，这表明它含有细胞内磷酸盐储存分子。作为Hfx。volcanii是多倍体，因此研究了DNA除了作为遗传物质的作用之外是否可以用作储存聚合物。这表明，在磷饥饿过程中，细胞既通过降解染色体，又通过分配染色体进行增殖。相比之下，核糖体的数量保持不变，表明核糖体分布到后代细胞中，但不降解。这些结果表明，含磷酸盐的生物分子（DNA和RNA除外）的磷酸盐来源于储存在DNA中的磷酸盐，而不是rRNA。向染色体缺失的细胞中加入磷酸盐可迅速恢复多倍性。不同倍性细胞脱水存活率的定量分析表明，在磷饥饿条件下，Hfx。volcanii减少了多倍体的遗传优势，有利于细胞增殖。使用基因组DNA作为磷酸盐储存聚合物的后果进行了讨论，以及假设，DNA可能最初演变的进化作为一个存储聚合物，和各种遗传学的好处进化后。
Haloferax volcanii uses extracellular DNA as a source for carbon, nitrogen, and phosphorous. However, it can also grow to a limited extend in the absence of added phosphorous, indicating that it contains an intracellular phosphate storage molecule. As Hfx. volcanii is polyploid, it was investigated whether DNA might be used as storage polymer, in addition to its role as genetic material. It could be verified that during phosphate starvation cells multiply by distributing as well as by degrading their chromosomes. In contrast, the number of ribosomes stayed constant, revealing that ribosomes are distributed to descendant cells, but not degraded. These results suggest that the phosphate of phosphate-containing biomolecules (other than DNA and RNA) originates from that stored in DNA, not in rRNA. Adding phosphate to chromosome depleted cells rapidly restores polyploidy. Quantification of desiccation survival of cells with different ploidy levels showed that under phosphate starvation Hfx. volcanii diminishes genetic advantages of polyploidy in favor of cell multiplication. The consequences of the usage of genomic DNA as phosphate storage polymer are discussed as well as the hypothesis that DNA might have initially evolved in evolution as a storage polymer, and the various genetic benefits evolved later.
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嗜盐古菌中受调控的多倍体。
影响因子：
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作者：
Breuert S;Allers T;Spohn G;Soppa J
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