Comparative study of modified quantitative buffy coat and two rapid tests in comparison with peripheral blood smear in malaria diagnosis in mumbai, India.
Comparative study of modified quantitative buffy coat and two rapid tests in comparison with peripheral blood smear in malaria diagnosis in mumbai, India.
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在印度孟买的疟疾诊断中，改进的定量Buffy涂层和两次快速测试的比较研究。
DOI：
10.1155/2014/194651
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发表时间：
2014
影响因子：
2.2
通讯作者：
Dasgupta D
中科院分区：
文献类型：
--
作者：
Kocharekar MM;Sarkar SS;Dasgupta D
为了确定一种快速可靠的技术，以准确诊断疟疾，研究的效率，如Parahit总（HRPII和醛缩酶Ag），优势mal卡（寄生虫特异性LDH），并与传统的血涂片显微镜检查进行比较。本研究包括100例感染间日疟原虫和101例感染恶性疟原虫的患者。Parahit total、Advantage mal卡和改良QBC检测恶性疟原虫的敏感性分别为70.3%、95%和98%，特异性分别为98%、98%和96%。Parahit total、Advantage mal卡和改良QBC检测间日疟原虫的敏感性分别为73%、97.0%和98%，特异性均为98%。第15天，恶性组Advantage mal-card和Parahit total分别出现8例（7.92%）和59例（58.41%）假阳性。在第15天，间日疟原虫组中，Parahit total显示52%的假阳性。研究表明，只有在有适当设施的情况下，才能使用经修改的QBC。Advantage mal卡是一种比Parahit total更好的随访工具。
In order to identify a quick and reliable technique for accurate diagnosis of malaria, study of the efficiency of the tests such as Parahit total (HRPII & aldolase Ag), Advantage mal card (parasite specific LDH), and modified QBC was done in comparison with conventional blood smear microscopy. One hundred patients infected with P. vivax and 101 infected with P. falciparum were included in this study. The sensitivity of Parahit total, Advantage mal card, and modified QBC for P. falciparum detection was 70.3, 95%, and 98%, and specificity was 98%, 98%, and 96%, respectively. The sensitivity of Parahit total, Advantage mal card, and modified QBC for P. vivax detection was 73%, 97.0%, and 98%, respectively, and specificity of all the tests was 98%. On day 15, in falciparum arm, Advantage mal card and Parahit total showed 8 (7.92%) and 59 (58.41%) false positives. On day 15, in vivax arm, Parahit total revealed 52% false positives. The study indicated that modified QBC could be only used where appropriate facilities are available. Advantage mal card was a better follow-up tool than Parahit total.