Identification of androgen-coregulated protein networks from the microsomes of human prostate cancer cells.
Identification of androgen-coregulated protein networks from the microsomes of human prostate cancer cells.
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DOI：
10.1186/gb-2003-5-1-r4
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发表时间：
2003
期刊：
影响因子：
12.3
通讯作者：
Aebersold R
中科院分区：
文献类型：
--
作者：
Wright ME;Eng J;Sherman J;Hockenbery DM;Nelson PS;Galitski T;Aebersold R
大规模的蛋白质组学方法已被用于定义LNCaP前列腺癌细胞中对雄激素治疗有反应的细胞过程。这些结果为雄激素在协调参与不同细胞过程的关键组分的表达中的作用提供了证据。雄激素在前列腺癌的发展中起着关键作用，雄激素调节的生长途径失调可导致前列腺癌。到目前为止，对雄激素调节的细胞过程还没有全面的了解。为此，我们应用了大规模的蛋白质组学方法来定义LNCaP前列腺癌细胞中对雄激素治疗有反应的细胞过程。使用同位素编码的亲和标签和质谱，我们确定并定量了1,064种蛋白质的相对丰度水平，发现不同的细胞过程受到雄激素的共同调节，而其他蛋白质基本上不受影响。随后的药理学扰动的细胞过程中产生的能量证实，雄激素饥饿有深刻的影响，这一途径。我们的研究结果为雄激素在协调参与不同细胞过程的关键组分的表达中的作用提供了证据，并进一步为前列腺癌细胞中雄激素调节蛋白网络和途径的全面重建奠定了基础。
A large-scale proteomic approach has been used to define cellular processes that are responsive to androgen treatment in LNCaP prostate cancer cells. The results provide evidence for the role of androgenic hormones in coordinating the expression of critical components involved in distinct cellular processes Androgens play a critical role in the development of prostate cancer-dysregulation of androgen-regulated growth pathways can led to hormone-refractory prostate cancer. A comprehensive understanding of androgen-regulated cellular processes has not been achieved to date. To this end, we have applied a large-scale proteomic approach to define cellular processes that are responsive to androgen treatment in LNCaP prostate cancer cells. Using isotope-coded affinity tags and mass spectrometry we identified and quantified the relative abundance levels of 1,064 proteins and found that distinct cellular processes were coregulated by androgen while others were essentially unaffected. Subsequent pharmacological perturbation of the cellular process for energy generation confirmed that androgen starvation had a profound effect on this pathway. Our results provide evidence for the role of androgenic hormones in coordinating the expression of critical components involved in distinct cellular processes and further establish a foundation for the comprehensive reconstruction of androgen-regulated protein networks and pathways in prostate cancer cells.