ANTIBODY-MEDIATED NEUTRALIZATION INVIVO OF INFECTIOUS PAPILLOMAVIRUSES
ANTIBODY-MEDIATED NEUTRALIZATION INVIVO OF INFECTIOUS PAPILLOMAVIRUSES
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DOI：
10.1128/jvi.64.7.3151-3156.1990
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发表时间：
1990-07-01
影响因子：
5.4
通讯作者：
KREIDER, JW
中科院分区：
文献类型：
--
作者：
CHRISTENSEN, ND;KREIDER, JW
特异性抗体介导的中和感染性人乳头瘤病毒11型（HPV-11）在无胸腺小鼠异种移植系统中实现，其中HPV-11诱导人包皮的形态转化。通过HPV-11特异性多克隆抗血清中和HPV-11感染性而非牛乳头瘤病毒1型（BPV-1）或棉尾兔乳头瘤病毒（CRPV）感染性的能力证明了病毒特异性中和。在所有三种病毒感染性系统中，通过病毒悬液未能诱导置于无胸腺小鼠肾囊下的适当皮肤异种移植物的形态学转化来检测中和。还针对BPV-1和CRPV的完整病毒体产生了兔多克隆抗血清，并在BPV-1和胎牛皮肤以及CRPV和兔耳皮肤的异种移植系统中测试了中和活性。三种多克隆抗血清含有病毒特异性中和抗体，表明中和表位存在于所有三种乳头瘤病毒上，并且这些表位是抗原性非交叉反应性的。无胸腺小鼠异种移植系统是检测乳头瘤病毒特异性中和抗体的有用模型，并提供了分析人乳头瘤病毒中和抗体的唯一机会。
Specific antibody-mediated neutralization of infectious human papillomavirus type 11 (HPV-11) was achieved in the athymic mouse xenograft system, in which HPV-11 induced morphological transformation of human foreskin. Virus-specific neutralization was demonstrated by the ability of an HPV-11-specific polyclonal antiserum to neutralize HPV-11 infectivity and not bovine papillomavirus type 1 (BPV-1) or cottontail rabbit papillomavirus (CRPV) infectivity. In all three virus infectivity systems, neutralization was detected by the failure of the virus suspension to induce morphological transformation of the appropriate skin xenografts placed under the renal capsule of athymic mice. Rabbit polyclonal antisera were also generated against intact virions of both BPV-1 and CRPV, and neutralizing activity was tested in the xenograft system with BPV-1 and fetal bovine skin and with CRPV and rabbit ear skin. The three polyclonal antisera contained virus-specific neutralizing antibodies, demonstrating that neutralizing epitopes existed on all three papillomaviruses and that these epitopes were antigenically non-cross-reactive. The athymic mouse xenograft system was a useful model for detecting papillomavirus-specific neutralizing antibodies and offers the only opportunity for the analysis of neutralizing antibodies to human papillomaviruses.