Hepcidin expression in the liver: Relatively low level in patients with chronic hepatitis C
Hepcidin expression in the liver: Relatively low level in patients with chronic hepatitis C
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DOI：
10.2119/2006-00057.fujita
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发表时间：
2007-01-01
影响因子：
5.7
通讯作者：
Kaito, Masahiko
中科院分区：
文献类型：
--
作者：
Fujita, Naoki;Sugimoto, Ryosuke;Kaito, Masahiko
慢性丙型肝炎患者常出现血清和肝脏铁超载，但其机制尚不清楚。最近发现的海普西丁，只在肝脏合成，被认为是铁稳态的关键调节因子，由感染和炎症诱导。本研究旨在检测不同肝病患者肝组织中海普西丁的表达水平。用实时荧光定量-聚合酶链式反应检测了56例丙型肝炎病毒(丙型肝炎病毒)阳性、34例乙肝病毒(乙肝病毒)阳性、42例丙型肝炎病毒和乙肝病毒阴性的肝组织标本中海普西丁基因的表达水平。我们分析了海普西丁与临床、血液学、组织学和病因学结果的关系。海普西丁的表达水平与血清铁蛋白(P<；0.0001)和肝组织铁沉积程度(P<；0,0001)密切相关。海普西丁还与血液学参数(与血红蛋白相比，P=0.0073；与血清铁，P=0.0012；与转铁蛋白饱和度，P<；0.0001)和转氨酶水平(P=0.0013)相关。丙型肝炎病毒阳性患者的肝素/铁蛋白比值显著低于乙肝病毒阳性患者(P=0.0129)和正常对照组(P=0.0080)。综上所述，慢性肝病患者的海普西丁表达水平与血清铁蛋白浓度或肝脏铁沉积程度密切相关。经血清铁蛋白水平或肝铁评分调整后，丙型肝炎病毒阳性患者的肝铁蛋白指数显著低于乙肝病毒阳性患者，提示其可能在慢性丙型肝炎患者铁负荷过高的发病机制中起关键作用。
Patients with chronic hepatitis C frequently have serum and hepatic iron overload, but the mechanism is unknown. Recently identified hepcidin, exclusively synthesized in the liver, is thought to be a key regulator for iron homeostasis and is induced by infection and inflammation. This study was conducted to determine the hepatic hepcidin expression levels in patients with various liver diseases. We investigated hepcidin mRNA levels of liver samples by real-time detection-polymerase chain reaction; 56 were hepatitis C virus (HCV) positive, 34 were hepatitis B virus (HBV) positive, and 42 were negative for HCV and HBV (3 cases of autoimmune hepatitis, 7 alcoholic liver disease, 13 primary biliary cirrhosis, 9 nonalcoholic fatty liver disease, and 10 normal liver). We analyzed the relation of hepcidin to clinical, hematological, histological, and etiological findings. Hepcidin expression levels were strongly correlated with serum ferritin (P < 0.0001) and the degree of iron deposit in liver tissues (P < 0,0001). Hepcidin was also correlated with hematological parameters (vs. hemoglobin, P = 0.0073; vs. serum iron, P = 0.0012; vs. transferrin saturation, P < 0.0001) and transaminase levels (P = 0.00 13). The hepcidin-to-ferritin ratio was significantly lower in HCV+ patients than in HBV+ patients (P = 0.0129) or control subjects (P = 0.0080). In conclusion, hepcidin expression levels in chronic liver diseases were strongly correlated with either the serum ferritin concentration or degree of iron deposits in the liver. When adjusted by either serum ferritin values or hepatic iron scores, hepcidin indices were significantly lower in HCV+ patients than in HBV+ patients, suggesting that hepcidin may play a pivotal role in the pathogenesis of iron overload in patients with chronic hepatitis C.