Glyceraldehyde-3-phosphate dehydrogenase gene over expression correlates with poor prognosis in non small cell lung cancer patients.
Glyceraldehyde-3-phosphate dehydrogenase gene over expression correlates with poor prognosis in non small cell lung cancer patients.
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DOI：
10.1186/1476-4598-12-97
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发表时间：
2013-08-29
期刊：
影响因子：
37.3
通讯作者：
Pfeffer U
中科院分区：
文献类型：
--
作者：
Puzone R;Savarino G;Salvi S;Dal Bello MG;Barletta G;Genova C;Rijavec E;Sini C;Esposito AI;Ratto GB;Truini M;Grossi F;Pfeffer U
已知糖酵解是许多肿瘤中选择的代谢，在氧气存在下具有高葡萄糖消耗。在肺癌中，这种现象通常被用于氟脱氧葡萄糖摄取的诊断PET成像，但对切除的肺肿瘤样本中糖酵解水平评估的预后能力知之甚少。在这项回顾性研究中，我们使用真实的时间聚合酶链反应（RQ-PCR）来评估82例连续早期切除的肿瘤样本中3-磷酸甘油醛脱氢酶（GAPDH）（葡萄糖分解的关键酶）基因的表达水平。非小细胞肺癌（NSCLC）患者。然后，我们将我们的结果与六个大型公开的NSCLC微阵列数据集进行了比较，这些数据集收集了1250多名患者的数据。在我们的研究中发现GAPDH基因过表达是早期NSCLC的不良预后因素（n = 82 HR = 1.30 p = 0.050）。该结果在分析的6个公共数据集中的5个中得到证实：Shedden等人，2008年：n = 442 HR = 1.54 p < 0.0001; Lee等人，2008年：n = 138 HR = 1.31 p = 0.043; Tomida等人，2009年：n = 117 HR = 1.59 p = 0.004; Roepman等人，2009年：n = 172（TPI 1基因）HR = 1.51 p = 0.009;冈山等人2012：n = 226 HR = 3.19 p < 0.0001; Botling等人2013：n = 196 HR = 1.00 p = 0.97）。此外，在大型且临床上充分注释的Shedden等人的微阵列数据集中，GAPDH风险比没有改变，无论是针对整个数据集还是仅针对佐剂初治患者亚组计算（n = 330 HR = 1.49 p < 0.0001）。GAPDH基因在非小细胞肺癌切除标本中的过度表达是一个不良的预后因素。我们的结果证实了糖代谢评估在NSCLC中的预后价值。
Glycolysis in presence of oxygen with high glucose consumption is known to be the metabolism of choice in many tumors. In lung cancer this phenomenon is routinely exploited in diagnostic PET imaging of fluorodeoxyglucose uptake, but not much is known about the prognostic capabilities of glycolysis level assessment in resected lung tumor samples. In this retrospective study, we used real time polymerase chain reaction(RQ-PCR) to assess the expression level of the gene for Glyceraldehyde 3-phosphate dehydrogenase(GAPDH), key enzyme for glucose breakdown, in tumor samples from 82 consecutive early stages resected non small cell lung cancer(NSCLC) patients. We then compared our results in six large publicly available NSCLC microarray datasets collecting data from over 1250 total patients. In our study GAPDH gene over expression was found to be an adverse prognostic factor in early stages NSCLC (n = 82 HR = 1.30 p = 0.050). This result was confirmed in 5 of 6 public datasets analyzed: Shedden et al. 2008: n = 442 HR = 1.54 p < 0.0001; Lee et al. 2008: n = 138 HR = 1.31 p = 0.043; Tomida et al. 2009: n = 117 HR = 1.59 p = 0.004; Roepman et al. 2009: n = 172 (TPI1 gene) HR = 1.51 p = 0.009; Okayama et al. 2012: n = 226 HR = 3.19 p < 0.0001; Botling et al. 2013: n = 196 HR = 1.00 p = 0.97). Furthermore, in the large and clinically well annotated Shedden et al. microarray dataset, GAPDH hazard ratio did not change whether calculated for the whole dataset or for the subgroup of adjuvant naive patients only (n = 330 HR = 1.49 p < 0.0001). GAPDH gene over expression in resected tumor samples is an adverse prognostic factor in NSCLC. Our results confirm the prognostic value of glucose metabolism assessment in NSCLC.
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影响因子：
11.2
作者：
Nair VS;Gevaert O;Davidzon G;Napel S;Graves EE;Hoang CD;Shrager JB;Quon A;Rubin DL;Plevritis SK
通讯作者：
Plevritis SK
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
45.3
作者：
Pignon, Jean-Pierre;Tribodet, Helene;Le Chevalier, Thierry
通讯作者：
Le Chevalier, Thierry
影响因子：
9
作者：
Hyun, Seung Hyup;Choi, Joon Young;Kim, Byung-Tae
通讯作者：
Kim, Byung-Tae
影响因子：
11.5
作者：
Roepman, Paul;Jassem, Jacek;van Zandwijk, Nico
通讯作者：
van Zandwijk, Nico