Intralobular heterogeneity of oxidative stress and cell death in ischemia-reperfused rat liver
Intralobular heterogeneity of oxidative stress and cell death in ischemia-reperfused rat liver
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DOI：
10.1006/jsre.2000.5831
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发表时间：
2001-02-01
影响因子：
2.2
通讯作者：
Koyama, K
中科院分区：
文献类型：
--
作者：
Kato, Y;Tanaka, J;Koyama, K
本文研究了大鼠肝脏缺血再灌注损伤后氧化应激与细胞死亡的关系。氧化应激通过灌注硝基蓝四唑原位检测，硝基蓝四唑通过还原剂转化为不溶性蓝甲瓒。通过台盼蓝灌注原位检测细胞死亡。当离体肝脏热缺血30或60分钟后，再灌注30分钟后，门静脉周围的实质细胞中检测到氧化应激。再灌注60分钟后，它以甜甜圈的形状扩散到中间带细胞。另一方面，在检测到氧化应激的甜甜圈样区域内的实质细胞中观察到细胞死亡。缺血60 min后氧化应激和细胞死亡的程度高于缺血30 min后。在非实质细胞中，氧化应激仅在12分钟的再灌注后，在中间带和中央周围区域观察到，但轻微的细胞死亡仅在门静脉周围和中间带区域再灌注30分钟后观察到。别嘌呤醇，黄嘌呤氧化酶抑制剂，抑制门静脉周围实质细胞的氧化应激和细胞死亡。这些结果表明，门静脉周围和中间带实质细胞死亡可以引起的区域特异性和黄嘌呤氧化酶介导的氧化应激在实质细胞。(C)北京：科学出版社.
The relationship between zonal oxidative stress and cell death after ischemia-reperfusion injury in rat liver was investigated. Oxidative stress was detected in situ by perfusion with nitroblue tetrazolium, which is converted to insoluble blue formazan by reducting agents. Cell death was detected in situ by perfusion with trypan blue. When isolated liver was perfused after 30 or 60 min of warm ischemia, oxidative stress was detected in periportal parenchymal cells after 30 min of reperfusion. It spread in the shape of a doughnut to midzonal cells after 60 min of reperfusion. On the other hand, cell death was observed in parenchymal cells that were within the doughnut-like area in which oxidative stress was detected. The extent of oxidative stress and cell death was higher after 60 min of ischemia than after 30 min of ischemia. In nonparenchymal cells, oxidative stress was observed in midzonal and pericentral regions after only 12 min of reperfusion, but minor cell death was observed only in periportal and midzonal regions after 30 min of reperfusion. Administration of allopurinol, an inhibitor of xanthine oxidase, suppressed oxidative stress and cell death in periportal parenchymal cells. These findings indicate that periportal and midzonal parenchymal cell death can be caused by zone-specific and xanthine-oxidase-mediated oxidative stress in parenchymal cells. (C) 2001 Academic Press.