[Evaluation of application of a new molecular marker SRAP on analysis of F2 segregation population and genetic diversity in cotton].
[Evaluation of application of a new molecular marker SRAP on analysis of F2 segregation population and genetic diversity in cotton].
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发表时间：
2004-06
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通讯作者：
Zhongxu Lin;Xianlong Zhang;Y. Nie
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作者：
Zhongxu Lin;Xianlong Zhang;Y. Nie
将序列相关扩增多态(SRAP)这一新的标记体系应用于棉花基因组分析。建立了一套可靠、有效、重复性好的检测SRAP的高效的PCR反应体系。利用SRAP标记检测了‘Pima90’(海岛棉)和‘Handan208’(陆地棉)之间的遗传多样性，表现出较高的多态性。在30对引物中，29对共扩增出149条多态性条带，平均每对扩增出5.14条多态性条带。其中以ME3和em2组合的多态性最高，共扩增出13条多态条带。对11个陆地棉品种进行了SRAP标记检测，30对引物中有15对表现出多态性，共扩增出22个多态位点，在陆地棉品种中表现较高。结果表明，SRAP标记可广泛应用于棉花分子遗传图谱构建和种质资源评价。
SRAP (Sequence-related Amplified Polymorphism), a new marker system, was applied in cotton genome analysis. We developed an efficient PCR reaction system for detecting SRAP that showed reliable, effective and reproductive. SRAP marker was used to detect the polymorphisms between 'Pima90' (G. barbodense) and ' Handan208' (G. hirsutum) and showed comparatively high polymorphism. Among the 30 primer pairs, 29 pairs generated 149 polymorphic bands with an average of 5.14 polymorphic bands per primer pair. The primer pair showing most polymorphism was the combination of me3 and em2, which produced 13 polymorphic bands. The SRAP marker was also tested among 11 upland cotton cultivars, 15 of the tested 30 primer pairs showed polymorphism yielding 22 polymorphic genetic loci, which is relatively high among upland cotton cultivars. The results showed that SRAP marker could be widely used in molecular genetic map construction and germplasm evaluation of cotton.