ENHANCED SPINAL-CORD REGENERATION IN LAMPREY BY APPLIED ELECTRIC-FIELDS
ENHANCED SPINAL-CORD REGENERATION IN LAMPREY BY APPLIED ELECTRIC-FIELDS
复制标题
DOI：
10.1126/science.7256258
复制
发表时间：
1981-01-01
期刊：
影响因子：
56.9
通讯作者：
COHEN, MJ
中科院分区：
文献类型：
--
作者：
BORGENS, RB;ROEDERER, E;COHEN, MJ
经过大约10亩的微弱稳定电流。在完全切断的七鳃鳗幼鱼脊髓上施加A，在切断的巨大网状脊髓神经元中观察到增强的再生。横断后（阴极离病灶远端）用植入芯电极施加电流5-6天。术后44 ~ 63 d采用细胞内荧光染色及电生理检查脊髓。整个脊髓的细胞外刺激显示，在大多数电处理的准备中，动作电位在病变的两个方向上传导，但在大多数假处理的对照组中，它们没有在任何一个方向上传导。在大多数电治疗动物,流程从巨大的轴突肿胀的不规则的提示,显示活跃的增长,被认为或病变。只有少数经过假治疗的对照组表现出这些特征。这些促进的再生反应可能是由人工施加的电场对进入脊髓病变的自然稳定损伤电流的影响介导的。
After a weak, steady electric current of approximately 10 .mu.A was imposed across the completely severed spinal cord of the larval lamprey P. marinus, enhanced regeneration was observed in the severed giant reticulospinal neurons. The current was applied with implanted wick electrodes for 5-6 days after transection (cathode distal to lesion). The spinal cords were examined 44-63 days after the operation by means of intracellular fluorescent dye injections and electrophysiology. Extracellular stimulation of whole cords showed that action potentials in most of the electrically treated preparations were conducted in both directions across the lesion, but they were not conducted in either direction in most of the sham-treated controls. In most of the electrically treated animals, processes from giant axons with swollen irregular tips, indicating active growth, were seen in or across the lesion. Only a few of the sham-treated controls showed these features. These facilitated regenerative responses were possibly mediated by the effects of the artificially applied electric fields on the natural steady current of injury entering the spinal lesion.