Hepatozoon canis infection in Slovakia:: Imported or autochthonous?
Hepatozoon canis infection in Slovakia:: Imported or autochthonous?
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DOI：
10.1089/vbz.2006.0598
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发表时间：
2007-06-01
影响因子：
2.1
通讯作者：
Petko, Branislav
中科院分区：
文献类型：
--
作者：
Majlathova, Viktoria;Hurnikova, Zuzana;Petko, Branislav
采用PCR方法检测了来自米哈洛夫采地区（斯洛伐克）的9只赤狐（4份横纹肌组织和5份心脏组织）的组织样本，该地区是犬巴贝虫病的地方性发生区，使用引物扩增跨越巴贝虫和泰勒虫V4区的18 S rRNA片段。在4份样品中发现了犬肝吸虫450 bp DNA片段的意外测定。本文报道了18SrRNA基因的部分序列。与巴西分离株的同源性为100%。来自潘帕斯草原狐（Pseudalopex gymnocercus）（AY 471615）以及来自西班牙的狐（AY 150067）和来自巴西的狗（AY 864677）的犬。在本研究中，我们报告的第一个PCR检测犬肝体在自然感染的红狐从斯洛伐克，一个Rhipicephalus sanguineus自由的地区。我们假设感染是由感染的R.这些红腹野鼠可能是由旅行者、金豺或狐狸带到斯洛伐克的，因为金豺的扩张以及环境和气候的变化。
Tissue samples from nine red foxes (four samples of striated muscle tissue and five samples of heart tissue) that originated from the Michalovce district (Slovakia), an area with endemic occurrence of canine babesiosis were examined by PCR method using primers amplifying a fragment of the 18S rRNA spanning the V4 region of Babesia and Theileria. An unexpected determination of 450 bp DNA fragment of Hepatozoon canis was found in four samples. Partial sequences of the 18S rRNA gene from the H. canis showed 100% similarity with the sequence from Brasil isolate of H. canis from a pampas fox (Pseudalopex gymnocercus) (AY471615) as well as from a fox in Spain (AY150067) and from a dog in Brazil (AY864677). In the present study, we report the first PCR detection of Hepatozoon canis in a naturally infected red fox from Slovakia, a Rhipicephalus sanguineus-free region. We assume that the infection was spread by infected R. sanguineus that might have been brought to Slovakia by travelers, by golden jackals, or by foxes migrating because of expansion of golden jackals and environmental and climate changes.