Comparative genomics provide a rapid detection of Fusarium oxysporum f. sp conglutinans
Comparative genomics provide a rapid detection of Fusarium oxysporum f. sp conglutinans
复制标题
比较基因组学提供了尖镰刀菌 f 的快速检测。
DOI：
10.1016/s2095-3119(15)61237-0
复制
发表时间：
2016-01-01
影响因子：
4.8
通讯作者：
Yang Yu-hong
中科院分区：
文献类型：
--
作者：
Ling Jian;Zhang Ji-xiang;Yang Yu-hong
香蕉枯萎甘蓝枯萎病菌（Fusarium wilt disease）是甘蓝枯萎病的病原菌。一种快速、准确、可靠的植物病原菌检测和鉴定方法对植物病害的综合治理至关重要。在这项研究中，我们利用尖孢镰刀菌（Fo）的比较基因组分析，开发了Foc特异性引物组（Focs-1/Focs-2）并建立了多重PCR检测方法。Focs-1/Focs-2和镰刀菌通用引物（W106 R/F106 S）可在一个PCR反应中检测Foc菌株。在优化的PCR条件下，多重PCR检测Foc具有较高的特异性，检测灵敏度可达100 pg Foc基因组DNA或1 g二次高压灭菌土壤中1 000个孢子。我们还表明，Foc菌株很容易检测到从受感染的植物组织，以及从自然领域的土壤，使用多重PCR检测。据我们所知，这是第一个报告，检测Fo的比较基因组方法。
Fusarium oxysporum f. sp. conglutinans (Foc) is the causal agent of Fusarium wilt disease of Brassica oleracea. A rapid, accurate, and reliable method to detect and identify plant pathogens is vitally important to integrated disease management. In this study, using a comparative genome analysis among Fusarium oxysporum (Fo), we developed a Foc-specific primer set (Focs-1/Focs-2) and established a multiplex-PCR assay. In the assay, the Focs-1/Focs-2 and universal primers for Fusarium species (W106R/F106S) could be used to detect Foc isolates in a single PCR reaction. With the optimized PCR parameters, the multiplex-PCR assay showed a high specificity for detecting Foc and was very sensitive to detect as little as 100 pg of pure Foc genomic DNA or 1 000 spores in 1 g of twice-autoclaved soil. We also demonstrated that Foc isolates were easily detected from infected plant tissues, as well as from natural field soils, using the multiplex-PCR assay. To our knowledge, this is a first report on detection Fo by comparative genomic method.