The clearance and degradation of oligodeoxynucleotides following intravenous injection into rabbits.
The clearance and degradation of oligodeoxynucleotides following intravenous injection into rabbits.
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静脉注射兔子后寡脱氧核苷酸的清除和降解。
DOI：
10.1089/ard.1991.1.153
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Zamecnik,PC
中科院分区：
文献类型：
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作者：
Goodchild,J;Kim,B;Zamecnik,PC
发现 A32P 标记的 20 个碱基的寡脱氧核苷酸在新鲜抽取的兔或人血液中相当稳定，并且在 2 小时内没有明显的降解。相比之下，注射到兔子体内后，相同的寡脱氧核苷酸迅速从血液中清除，并且至少 17% 在 5 分钟内完全降解。 90 分钟后，尿液中发现 16% 的寡核苷酸基本完整。此时，在所有测试的实体组织中也发现了放射性同位素，但可能不是完整的寡核苷酸。
A32P-labeled oligodeoxynucleotide of 20 bases was found to be quite stable in freshly drawn rabbit or human blood and no degradation was apparent within 2 hr. In contrast, following injection into rabbits, the same oligodeoxynucleotide was cleared from the blood rapidly and at least 17% was completely degraded within 5 min. After 90 min, 16% of the oligonucleotide was found largely intact in the urine. At this time, radioisotope was also found in all the solid tissues tested but probably not as intact oligonucleotide.