Highly dispersed magnetic molecularly imprinted nanoparticles with well-defined thin film for the selective extraction of glycoprotein
Highly dispersed magnetic molecularly imprinted nanoparticles with well-defined thin film for the selective extraction of glycoprotein
复制标题
具有明确薄膜的高度分散的磁性分子印迹纳米粒子，用于选择性提取糖蛋白
DOI：
10.1039/c6tb00409a
复制
发表时间：
2016-01-01
影响因子：
7
通讯作者：
Shi, Yan-Ping
中科院分区：
文献类型：
--
作者：
Ma, Run-Tian;Ha, Wei;Shi, Yan-Ping
无抗体分析是糖蛋白分析的一种潜在方法，但近年来该方法的发展因其不利的选择性而受到限制。磁性分子印迹结合了磁性材料的快速分离和分子印迹中对模板的高选择性，有望成为糖蛋白无抗体分析中有效的样品预处理方法。然而，磁性印迹纳米粒子和厚的分子印迹聚合物（MIP）壳在磁性载体表面的聚集导致了不利的吸附能力以及不理想的重结合和洗脱率，限制了其在糖蛋白提取中的应用。因此，本工作开发了具有明确薄膜的高度分散的磁性分子印迹纳米颗粒（MMIN），用于选择性提取糖蛋白 HRP。本工作采用溶剂热法来提高 Fe3O4 NPs（纳米颗粒）和 MMINs 的分散性。 MIP 薄膜的厚度经过优化，可提供最佳的提取效率。从而大大提高了MMINs的吸附能力、模板的重结合率和洗脱率。因此，制备的 MMIN 不仅对 HRP 表现出优异的选择性和高吸附能力，以及出色的抗干扰能力，而且在提取应用中表现出优异的再结合率和洗脱率。此外，该方法为改进传统磁性分子印迹提供了可靠的方法，并在未来临床糖蛋白肿瘤生物标志物分析中显示出巨大的潜力。
Antibody-free analysis is a potential method for glycoprotein analysis, but the development of this method has been limited by its unfavorable selectivity in recent years. Magnetic molecular imprinting, which integrates the fast separation of magnetic materials with high selectivity towards templates in molecular imprinting, was expected to be an effective sample pretreatment in antibody-free analysis for glycoproteins. However, the aggregation of magnetic imprinted nanoparticles and thick molecularly imprinted polymer (MIP) shells on the surface of magnetic carriers caused an unfavorable adsorption capacity, and unsatisfactory rebinding and elution rates, and has limited its application in glycoprotein extraction. Thus, highly dispersed magnetic molecularly imprinted nanoparticles (MMINs) with a well-defined thin film for the selective extraction of glycoprotein HRP were developed in this work. A solvothermal method was used in this work to improve the dispersity of Fe3O4 NPs (nanoparticles) and the MMINs. The thickness of the MIP film was optimized to provide the optimum extraction efficiency. Thus the adsorption capacity of the MMINs, the rebinding rate and the elution rate of the templates were greatly improved. As a result, the prepared MMINs not only exhibited excellent selectivity and high adsorption capacity to HRP, and an outstanding tolerance for interference, but also showed excellent rebinding and elution rates for extraction application. Furthermore, this method provided a reliable way to improve conventional magnetic molecular imprinting, and showed great potential for the analysis of glycoprotein tumor biomarkers in clinics in the future.