Lipid transfer protein from Manduca sexta hemolymph.
Lipid transfer protein from Manduca sexta hemolymph.
复制标题
来自 Manduca sexta 血淋巴的脂质转移蛋白。
DOI：
10.1016/0006-291x(86)90903-4
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发表时间：
1986
影响因子：
3.1
通讯作者：
Law,JH
中科院分区：
文献类型：
--
作者：
Ryan,RO;Wells,MA;Law,JH
从烟草天蛾Manducasexta血淋巴中分离到一种脂转移蛋白（LTP）。LTP在体外催化分离的血淋巴脂蛋白之间的净脂质转移。采用密度梯度超离心和凝胶渗透色谱法的分离程序产生纯化的蛋白质。LTP是一种极高密度脂蛋白，其颗粒M r> 500，000。十二烷基硫酸钠聚丙烯酰胺凝胶电泳显示LTP由两种脱辅基蛋白组成：apoLTP-Ⅰ（Mr 320，000）和apoLTP-Ⅱ（Mr 85，000）。LTP可能在改变主要血淋巴脂蛋白（lipophorin）的脂质含量和组成方面具有生理作用。
A hemolymph lipid transfer protein (LTP) was isolated from the tobacco hornworm, Manduca sexta. LTP catalyzes net lipid transfer between isolated hemolymph lipoproteins in vitro. An isolation procedure employing density gradient ultracentrifugation and gel permeation chromatography produced a purified protein. LTP is a very high density lipoprotein with a particle M r> 500,000. Sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed that LTP is comprised of two apoproteins: apoLTP-I (M r∼ 320,000) and apoLTP-II (M r∼ 85,000). LTP may have a physiological role in altering the lipid content and composition of the major hemolymph lipoprotein, lipophorin.