A preliminary study of the anti-cancer effect of tanshinone on hepatic cancer and its mechanism of action in mice
A preliminary study of the anti-cancer effect of tanshinone on hepatic cancer and its mechanism of action in mice
复制标题
DOI：
10.1007/bf02954016
复制
发表时间：
1996-11
影响因子：
5.1
通讯作者：
Xiujie Wang;S. Yuan;Chao-jun Wang;Renmin Huang;Yuqiong Li
中科院分区：
文献类型：
--
作者：
Xiujie Wang;S. Yuan;Chao-jun Wang;Renmin Huang;Yuqiong Li
目的：通过体外实验研究，证实丹参素对某些肿瘤细胞具有细胞毒活性。但在体内尚未见抑癌活性的报道。本实验旨在证实其体内抗癌活性。方法：采用DMSO、5-Fu、Tan处理肝癌h22小鼠，实验结束后处死小鼠，分离肿瘤组织称重，计算抑瘤率，重复3次相同实验。采用活体溴脱氧尿苷标记法和肿瘤组织增殖细胞核抗原（PCNA）免疫组化染色法检测小鼠肝癌h22细胞的增殖动力学。结果：3个实验中，Tan对肿瘤的抑制率分别为50.0%、38.5%、40.6%，与dmso治疗对照组比较，差异均有统计学意义（P<0.01）。Brdu标记阳性细胞为51.8±7.9，PCNA阳性细胞为451.1±26.1，显著低于对照组（84.4±24.3,694.8±117.1）（P<0.01）。结论：丹参对体内肝癌有抗肿瘤作用；其作用机制可能与抑制肿瘤细胞的DNA合成、PCNA表达和DNA聚合酶δ活性有关。
Objective: There were some experimental researchesin vitro, which showed that tanshinonoe (Tan) had cytotoxic activities against some cancer cell lines. But there was no report of anti-cancer activity of Tanin vivo. This experimental study was performed to confirm the anti-cancer activity of Tanin vivo. Methods: Hepatic carcinoma H22bearing mice were treated with DMSO, 5-Fu, and Tan, at the end of experiment, the mice were sacrificed, tumor tissues were separated and weighed, and the tumor inhibitory rate was calculated, 3 times of the same experiments were performed. The proliferating kinetics of hepatic carcinoma H22cells in mice was measured by bromodeoxyuridine labelingin vivoand immunohistochemical staining of the proliferating cell nuclear antigen (PCNA) in tumor tissues. Results: The tumor inhibitory rates of Tan were 50.0%, 38.5%, and 40.6% in 3 experiments, respectively, compared with those of the DMSO-treated control groups, the differences were significant statistically (P<0.01). The Brdu labeling and PCNA positive cells were 51.8±7.9 and 451.1±26.1, respectively, which were significantly lower than those of controls (84.4±24.3, 694.8±117.1) (P<0.01). Conclusion: Tan had anti-cancer effect on hepatic carcinomain vivo; The mechanisms of action might be associated with inhibition of DNA synthesis, PCNA expression and DNA polymerase δ activity of tumor cells.