Antisera of designed specificity for subunits of guanine nucleotide-binding regulatory proteins.
Antisera of designed specificity for subunits of guanine nucleotide-binding regulatory proteins.
复制标题
针对鸟嘌呤核苷酸结合调节蛋白亚基设计的特异性抗血清。
DOI：
10.1073/pnas.83.2.265
复制
发表时间：
1986
影响因子：
11.1
通讯作者：
Gilman,AG
中科院分区：
文献类型：
--
作者：
Mumby,SM;Kahn,RA;Manning,DR;Gilman,AG
Antisera were raised against purified subunits of regulatory GTP-binding proteins (G proteins) and against synthetic peptides that correspond to defined regions of G proteins. Peptide antisera were generated that recognized all alpha or all beta subunits from Gs, Gi, Go, and transducin; others recognized only Gs alpha or Go alpha. Such cross-reaction or complete specificity for a given alpha subunit was not obtained when purified subunits were injected. Peptide antisera were used to identify G protein subunits in selected tissue membrane preparations by immunoblots.
