Feasibility of lettuce cultivation in sophoroliplid-enhanced washed soil originally polluted with Cd, antibiotics, and antibiotic-resistant genes
Feasibility of lettuce cultivation in sophoroliplid-enhanced washed soil originally polluted with Cd, antibiotics, and antibiotic-resistant genes
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槐糖脂强化水洗土壤种植生菜的Cd、抗生素和抗生素抗性基因污染的可行性
DOI：
10.1016/j.ecoenv.2015.11.013
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发表时间：
2016
影响因子：
6.8
通讯作者：
Jiang Xin
中科院分区：
文献类型：
--
作者：
Ye Mao;Sun Mingming;Wan Jinzhong;Feng Yanfang;Zhao Yu;Tian Da;Hu Feng;Jiang Xin
在重金属、抗生素和高丰度的抗虫基因（ARGs）污染的土壤中种植蔬菜，可通过食物链严重威胁人类健康。因此，迫切需要既能修复土壤又能确保粮食安全的新技术。在本研究中，用20 g L-1槐内酯溶液连续两次洗涤加超声（35 kHz）可有效提取71.2%的镉、88.2%的四环素、96.6%的磺胺嘧啶和100%的罗红霉素。同时，ARG（tetM、tetX、sulI和sulII）的相对丰度降低到10−7-10−8（ARG拷贝/16 S拷贝）。此外，在第二次洗涤的土壤中的莴苣栽培显示出蔬菜生长指数（鲜/干重、根表面积、叶绿素含量和可溶性蛋白质含量）的显著改善，以及莴苣组织中的抗逆细菌内生菌的分离物计数和ARG丰度的降低。这种组合的清理策略提供了一种环境友好的技术，以确保蔬菜在洗涤土壤中的安全性。
Vegetable cultivation in soils polluted with heavy metals, antibiotics and a high abundance of antibiotic-resistance genes (ARGs) can seriously threaten human health through the food chain. Therefore, novel techniques that not only remediate soil, but also ensure food security are urgently required. In the present study, two successive washings with 20 g L−1of sophoroliplid solution plus ultrasonication (35 kHz) were effective in extracting 71.2% Cd, 88.2% tetracycline, 96.6% sulfadiazine, and 100% roxithromycin. Simultaneously, relative abundance of ARGs (tetM,tetX,sulI, andsulII) was decreased to 10−7–10−8(ARG copies/16S copies). Further, lettuce cultivation in the 2nd washed soil showed significant improvement in vegetable growth indices (fresh/dry weight, root surface area, chlorophyll content and soluble protein content) and a decrease in isolate counts for antibiotic-resistant bacterial endophytes and ARG abundance in lettuce tissues. This combined cleanup strategy provides an environmentally friendly technology for ensuring vegetable security in washed soils.