Molecularly imprinted polymers for separation of various sugars from human urine.
Molecularly imprinted polymers for separation of various sugars from human urine.
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DOI：
10.1016/j.talanta.2011.09.043
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发表时间：
2011-12
期刊：
影响因子：
6.1
通讯作者：
Burcu Okutucu;S. Onal
中科院分区：
文献类型：
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作者：
Burcu Okutucu;S. Onal
分子印迹聚合物是一种新型、简单、廉价的材料，可用于多种临床应用。苯硼酸作为功能单体常用于二醇的共价印迹。本研究合成了果糖、半乳糖、葡萄糖和棉子糖的苯硼酸酯，并以此为模板分析物。果糖、半乳糖和葡萄糖-苯硼酸印迹聚合物的吸附容量分别为75%、10%和30%。对所有糖印迹聚合物进行了批量再结合研究和Scatchard分析。葡萄糖是尿液中最常见的糖之一。葡萄糖：苯硼酸印迹聚合物用于分析葡萄糖，果糖，半乳糖，蔗糖，麦芽糖，乳糖和棉子糖在加标尿液。葡萄糖：苯硼酸印迹聚合物对尿中单糖的选择性为45-55%，对二糖和多糖的选择性为30- 35%。
Molecularly imprinted polymers were the new, simple and unexpensive materials that can be used in several clinical applications. Phenylboronic acid has been frequently used as functional monomer for the covalent imprinting of diols. In this study, the phenylboronic acid esters of fructose, galactose, glucose and raffinose were synthesized and then used as template analytes. The adsorption capacities of fructose, galactose and glucose–phenylboronic acid imprinted polymers were 75, 10 and 30%, respectively. The batch rebinding studies and Scatchard analysis were done for all sugar imprinted polymer. Glucose is one of the mostly found sugar in the urine. The glucose:phenylboronic acid imprinted polymer was used for the analysis of glucose, fructose, galactose, sucrose, maltose, lactose and raffinose in spiked urine. The selectivity of glucose:phenylboronic acid imprinted polymer to urine monosaccharides was found as nearly 45–55% and to di- and polysaccharides was found as 30–35%, respectively.