Crystallization and preliminary X-ray analysis of the reduced Rieske-type [2Fe-2S] ferredoxin derived from Pseudomonas sp strain KKS102
Crystallization and preliminary X-ray analysis of the reduced Rieske-type [2Fe-2S] ferredoxin derived from Pseudomonas sp strain KKS102
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DOI：
10.1107/s1744309107009992
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发表时间：
2007-04-01
影响因子：
0.9
通讯作者：
Senda, Toshiya
中科院分区：
文献类型：
--
作者：
Senda, Miki;Kishigami, Shinya;Senda, Toshiya
BphA3是Pseudomonas sp.菌株KKS102联苯双加氧酶BphA的rieske型[2Fe-2S]铁氧还蛋白组分，通过坐滴蒸汽扩散法在厌氧条件下结晶。晶体属于空间群P3(1)21，晶胞参数a = b = 49.6, c = 171.9埃，衍射分辨率为1.95埃。用氧化BphA3作为搜索模型进行分子置换计算，得到了满意的结果。
The reduced form of BphA3, a Rieske-type [2Fe-2S] ferredoxin component of the biphenyl dioxygenase BphA from Pseudomonas sp. strain KKS102, was crystallized by the sitting-drop vapour-diffusion method under anaerobic conditions. The crystal belongs to space group P3(1)21, with unit-cell parameters a = b = 49.6, c = 171.9 angstrom, and diffracts to a resolution of 1.95 angstrom. A molecular-replacement calculation using oxidized BphA3 as a search model yielded a satisfactory solution.