Increased expression of histamine H-1 receptor mRNA in allergic rhinitis
Increased expression of histamine H-1 receptor mRNA in allergic rhinitis
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DOI：
10.1111/j.1365-2222.1996.tb00553.x
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发表时间：
1996-04-01
影响因子：
6.1
通讯作者：
Sakakura, Y
中科院分区：
文献类型：
--
作者：
Iriyoshi, N;Takeuchi, K;Sakakura, Y
研究背景组胺通过组胺H-1受体（H（1）R）参与变应性鼻炎鼻部症状的发生。据报道，过敏性疾病患者引起打喷嚏的最低组胺浓度低于正常对照组。以往采用H（1）R结合试验对变应性鼻炎中H（1）R是否增加的研究存在分歧。方法从10例变应性鼻炎患者和10例正常人的下鼻甲粘膜刮片中提取RNA，控制对象。由于H（1）R基因缺乏内含子，我们用DNA酶处理RNA颗粒，以区分RNA和污染的基因组DNA。由于H（1）R和β-肌动蛋白mRNA的扩增在35个循环时仍处于指数期，因此通过逆转录-聚合酶链反应（RT-PCR）扩增H（1）R和β-肌动蛋白mRNA 35个循环。结果变应性鼻炎组和正常对照组的H（1）R/β-actin比值分别为0.88 ± 0.62和0.29 ± 0.17;结论变应性鼻炎患者鼻黏膜中H1 R mRNA表达增加。
Background Histamine plays an important role in producing nasal symptoms via histamine H-1 receptor (H(1)R) in allergic rhinitis. It is reported that the minimum histamine concentration that induces sneezing is lower in allergic patients than in normal control subjects. Previous studies by binding assay on H(1)R gave divided results on whether the number of H(1)Rs is increased in allergic rhinitis or not.Objective The objective of this study was to examine if H(1)R mRNA expression is increased in patients with allergic rhinitis compared with normal healthy volunteers.Methods We extracted RNA from scrapings of inferior turbinate mucosa of 10 patients suffering from allergic rhinitis and 10 control subjects. As the H(1)R gene lacks introns, we treated RNA pellets by DNase to distinguish RNA from contaminating genomic DNA. Since amplification of H(1)R and beta-actin mRNA remained in an exponential phase at 35 cycles, H(1)R and beta-actin mRNAs were amplified for 35 cycles by reverse transcription-polymerase chain reaction (RT-PCR). The PCR products were hybridized with internal probes and band intensities were quantitated by a densitometer.Results The mean +/- SD of H(1)R/beta-actin ratio was 0.88 +/- 0.62 for the patients with allergic rhinitis and 0.29 +/- 0.17 for the normal subjects; the difference was statistically significant (P < 0.01).Conclusions These data suggest that expression of H(1)R mRNA is increased in the nasal mucosa of the patients with allergic rhinitis.